MP36-14 MICRORNA-29B ATTENUATES FIBROSIS IN A RAT MODEL OF PEYRONIE’S DISEASE

Abstract

You have accessJournal of UrologyCME1 Apr 2023MP36-14 MICRORNA-29B ATTENUATES FIBROSIS IN A RAT MODEL OF PEYRONIE’S DISEASE Patrícia Candido, Caroline Chiovatto, Ruan Pimenta, Poliana Romão, Vitória Ghazarian, Juliana Camargo, Vanessa Guimarães, Gabriel Santos, Iran Silva, Bruno Nascimento, Jorge Hallak, Miguel Srougi, William Nahas, Nayara Viana, Katia Leite, Sabrina Reis, and Feres Camargo Maluf Patrícia CandidoPatrícia Candido More articles by this author , Caroline ChiovattoCaroline Chiovatto More articles by this author , Ruan PimentaRuan Pimenta More articles by this author , Poliana RomãoPoliana Romão More articles by this author , Vitória GhazarianVitória Ghazarian More articles by this author , Juliana CamargoJuliana Camargo More articles by this author , Vanessa GuimarãesVanessa Guimarães More articles by this author , Gabriel SantosGabriel Santos More articles by this author , Iran SilvaIran Silva More articles by this author , Bruno NascimentoBruno Nascimento More articles by this author , Jorge HallakJorge Hallak More articles by this author , Miguel SrougiMiguel Srougi More articles by this author , William NahasWilliam Nahas More articles by this author , Nayara VianaNayara Viana More articles by this author , Katia LeiteKatia Leite More articles by this author , Sabrina ReisSabrina Reis More articles by this author , and Feres Camargo MalufFeres Camargo Maluf More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003270.14AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Peyronie's disease (PD) is characterized by the formation of fibrotic plaques on the tunica albuginea (TA) of the penis. Effective treatments are limited, and surgery remains the best option, although it does not regress the disease. The therapy with microRNAs (miRNAs) is an attractive alternative for several pathologies because they act in the regulation of gene expression. The miRNA-29b regulates extracellular matrix genes, such as collagen, and its decreased expression can be found in fibrotic diseases. We aimed to investigate the therapeutic potential of miRNA-29b in a fibrin-induced rat model of PD. METHODS: To generate PD-like plaques, rats received injections into the TA at 100 µL of each of the human fibrin and thrombin solutions on days 0 and 5. After fibrin injections, 18 rats were randomly divided into three groups (n=6/group): PD+miRNA29b group, PD+miRNA negative group (Scramble), and sham group. Treatment with mimic miRNA-29b or its negative control was performed on days 14, 21, and 28. The mid-penile shaft was removed on day 30 for histologic examination by Hematoxylin and eosin (H&E), Masson trichrome, and Verhoeff staining and qPCR. RESULTS: The establishment of the animal model was confirmed by the histopathological analyses with the identification of fibrosis (Figure 1a-f), underexpression of miRNA-29b (Figure 1g) and overexpression of COL1A1 and TGF-β1 (Figure 1h-j). After treatment, the Fibrosis+miRNA-29b group showed a decreased fibrotic process in the TA (Figure 2a-i) and observed overexpression of miRNA-29b (Figure 2j) and underexpression of genes COL1A1, COL3A1, and TGF-β1 in the PD+miRNA29b group (Figure 2k-m). CONCLUSIONS: Here, we demonstrated for the first time that overexpression of miRNA-29b attenuated fibrosis in the rat model of PD. The results of this study suggest that treatment with miRNA-29b may represent a new effective tool for PD. Source of Funding: Not applicable © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e484 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Patrícia Candido More articles by this author Caroline Chiovatto More articles by this author Ruan Pimenta More articles by this author Poliana Romão More articles by this author Vitória Ghazarian More articles by this author Juliana Camargo More articles by this author Vanessa Guimarães More articles by this author Gabriel Santos More articles by this author Iran Silva More articles by this author Bruno Nascimento More articles by this author Jorge Hallak More articles by this author Miguel Srougi More articles by this author William Nahas More articles by this author Nayara Viana More articles by this author Katia Leite More articles by this author Sabrina Reis More articles by this author Feres Camargo Maluf More articles by this author Expand All Advertisement PDF downloadLoading ...