MP35-18 EXTRACORPOREAL SHOCKWAVE THERAPY IMPROVES CP/CPPS BY DOWN-REGULATING NLPR3 INFLAMMASOME IN A PROSTATITIS RAT MODEL

Abstract

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Prostate & Genitalia (MP35)1 Sep 2021MP35-18 EXTRACORPOREAL SHOCKWAVE THERAPY IMPROVES CP/CPPS BY DOWN-REGULATING NLPR3 INFLAMMASOME IN A PROSTATITIS RAT MODEL Dongho Shin, Yong Hyun Park, Hyuk Jin Cho, Usyn Ha, Sunghoo Hong, Ji Youl Lee, Sae Woong Kim, Sang Hoon Kim, Byung Il Yoon, and Woong Jin Bae Dongho ShinDongho Shin More articles by this author , Yong Hyun ParkYong Hyun Park More articles by this author , Hyuk Jin ChoHyuk Jin Cho More articles by this author , Usyn HaUsyn Ha More articles by this author , Sunghoo HongSunghoo Hong More articles by this author , Ji Youl LeeJi Youl Lee More articles by this author , Sae Woong KimSae Woong Kim More articles by this author , Sang Hoon KimSang Hoon Kim More articles by this author , Byung Il YoonByung Il Yoon More articles by this author , and Woong Jin BaeWoong Jin Bae More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002044.18AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: The aim of this study was to evaluate the anti-inflammatory and antioxidative effects of extracorporeal shockwave therapy (ESWT) on prostatitis and explore the mechanism. METHODS: RWPE-1 cells randomly divided into 5 groups: 1 RWPE-1 group (normal control), 2 LPS group (lipopolysaccharide inducing inflammation), 3 0.1ESWT group (treated by 0.1mJ/mm2 ESWT), 4 0.2ESWT group (treated by 0.2mJ/mm2 ESWT) and 5 0.3ESWT group (treated by 0.3mJ/mm2 ESWT). After ESWT administered, cells and supernatant were collected for ELISA and western blot. In vivo, Sprague-Dawley rats (n=36) were randomly divided into 3 groups: 1 normal group, 2 prostatitis group, and 3 ESWT group (12 for each). Prostatitis were induced by 17 beta-estradiol and dihydrotestosterone. 4 weeks after ESWT, pain index was assessed for all groups and prostates were collected for immunohistochemistry, immunofluorescence, apoptosis analysis and western blot. RESULTS: The optimal energy flux density of ESWT was 0.2 mJ/mm2 in vitro. ESWT ameliorated discomfort in rats with prostatitis. Inflammation was improved by ESWT in prostatitis rats (P<0.05). Compared to normal rats, overexpressed NLRP3 inflammasomes triggered apoptosis which was improved by ESWT in prostatitis (P<0.05). TLR4-NFκB pathway was overactive with prostatitis, compared to normal and ESWT group (P<0.05). And BAX/BAK pathway was inhibited by ESWT in prostatitis rats (P<0.05). CONCLUSIONS: ESWT improved CP/CPPS by reducing NLPR3 inflammasome and ameliorated apoptosis via inhibiting BAX/BAK pathway in a rat model. TLR4 might be the key protein bonding NLPR3 inflammasome and BAX/BAK pathway. ESWT should be a potential and promising treatment for CP/CPPS. Source of Funding: none © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e634-e634 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information Dongho Shin More articles by this author Yong Hyun Park More articles by this author Hyuk Jin Cho More articles by this author Usyn Ha More articles by this author Sunghoo Hong More articles by this author Ji Youl Lee More articles by this author Sae Woong Kim More articles by this author Sang Hoon Kim More articles by this author Byung Il Yoon More articles by this author Woong Jin Bae More articles by this author Expand All Advertisement Loading ...