MP35-09 TRANSPERINEAL PROSTATE BIOPSY: THE FIRST ANALYSIS OF THE TRUE PROSTATE MICROBIOME

Abstract

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Prostate & Genitalia (MP35)1 Sep 2021MP35-09 TRANSPERINEAL PROSTATE BIOPSY: THE FIRST ANALYSIS OF THE TRUE PROSTATE MICROBIOME Clay Martin, Pandya Shashank, Annette Lee, and Michael Schwartz Clay MartinClay Martin More articles by this author , Pandya ShashankPandya Shashank More articles by this author , Annette LeeAnnette Lee More articles by this author , and Michael SchwartzMichael Schwartz More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002044.09AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: The human microbiome has become a rapidly evolving field of research with health implications in a variety of organ systems. Despite this growing body of literature, little is understood about the microbiome of the prostate. A major difficulty in prostate microbiome research is the difficulty in uncontaminated sample collection. Prior studies have utilized transrectal biopsy cores or surgical pathology specimens, both of which introduce significant contamination. The shift from transrectal to transperineal prostate biopsy technique provides a novel way of obtaining uncontaminated prostate specimens. This study aims to use transperineal prostate biopsy specimens to provide an initial description of the microbiome of the prostate. METHODS: Adult, biopsy-naive men (age 18 and older) who were undergoing elective transperineal prostate biopsy for medically indicated purposes were collected prospectively for inclusion in the study. Those with prior prostate biopsies or surgery, a history of chronic or acute prostatitis, history of chronic pelvic pain, antibiotics within 30 days of biopsy, catheterization within 60 days of biopsy, history of chronic indwelling or clean intermittent catheterizations were excluded such to represent the native bacteria of the unmanipulated gland. In addition to 12-14 diagnostic biopsies, enrolled patients underwent 2 additional biopsies. These core biopsies, along with skin and urine samples were sent directly for microbiome analysis via Illumina 16S Metagenomic sequencing. RESULTS: Prostate tissue samples, voided urine and perineal skin swabs from 10 patients were sent for analysis. 1 prostate biopsy specimen was excluded for suspected contamination. Evidence of a distinct microbiome within the prostate was discovered. Prominent species included Pseudomonas veronii, Comamonas testosteroni and Achromobacter xylosoxidans groups. Phylogenic diversity and weighted UniFrac analysis suggest a significant difference between biopsy specimens and perineal skin swabs but not a significant difference between the biopsy specimens and voided urine samples. CONCLUSIONS: These findings suggest that there exists a distinct microbiome specific to the human prostate gland, which may be closely related to that of bladder urine. Sterile sampling via transperineal ultrasound-guided prostate biopsy represents a viable method of sample collection for microbiome analysis. Further research is needed to further characterize the microbiome of the prostate and to elucidate the potential implications on human health and disease states. Source of Funding: None © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e630-e630 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information Clay Martin More articles by this author Pandya Shashank More articles by this author Annette Lee More articles by this author Michael Schwartz More articles by this author Expand All Advertisement Loading ...