MP34-19 USE OF A MATRIX OF COLLAGEN AND ELASTIN TO BLADDER AUGMENTATION IN THE PORCINE MODEL

Abstract

You have accessJournal of UrologySurgical Technology & Simulation: Instrumentation & Technology III1 Apr 2016MP34-19 USE OF A MATRIX OF COLLAGEN AND ELASTIN TO BLADDER AUGMENTATION IN THE PORCINE MODEL Carlos Gasanz Serrano, Carles Xavier Raventos Busquets, Jordi Temprana Salvador, Lucas Regis Placido, Pol Servian Vives, Ricardo Lopez del Campo, Ignacio Arroyo Soto, Enric Miret Alomar, Merce Cuadras Soler, Luis Castro Sader, Cristian Konstantinidis Garay, Marielle Esteves Coelho, Carla Fonseca, Ines de Torres, and Juan Morote Robles Carlos Gasanz SerranoCarlos Gasanz Serrano More articles by this author , Carles Xavier Raventos BusquetsCarles Xavier Raventos Busquets More articles by this author , Jordi Temprana SalvadorJordi Temprana Salvador More articles by this author , Lucas Regis PlacidoLucas Regis Placido More articles by this author , Pol Servian VivesPol Servian Vives More articles by this author , Ricardo Lopez del CampoRicardo Lopez del Campo More articles by this author , Ignacio Arroyo SotoIgnacio Arroyo Soto More articles by this author , Enric Miret AlomarEnric Miret Alomar More articles by this author , Merce Cuadras SolerMerce Cuadras Soler More articles by this author , Luis Castro SaderLuis Castro Sader More articles by this author , Cristian Konstantinidis GarayCristian Konstantinidis Garay More articles by this author , Marielle Esteves CoelhoMarielle Esteves Coelho More articles by this author , Carla FonsecaCarla Fonseca More articles by this author , Ines de TorresInes de Torres More articles by this author , and Juan Morote RoblesJuan Morote Robles More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.1577AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The material used as the bladder substitute in the clinical practice is the intestine despite its high morbility (complex postoperatory, metabolic acidosis, lithogenesis, risk of neoplasia). The attempts to find another material have not been effective in everyday clinical practice. The aim of this study is to make a bladder augmentation with a matrix of collagen and elastin covered with an own flap of parietal peritoneum. We use a control group: a bladder augmentation only with a flap of parietal peritoneum. The aim is to find a material which can be used to create a new bladder wall instead of using the intestine. METHODS 11 female pigs were divided in two groups depending on the material used for the augmentation: matrix + peritoneum (8 pigs) and only peritoneum (3 pigs). The size of the augmentation was 5 x 5 cm approximately. The animals were monitored during 6 weeks. Afterwards, the pigs were euthanised and bladders were histologically examined: Hematoxilin-eosin, Gomori tricromic and Orcein stains. In addition, we practiced a single-channel cystometry just before the augmentation and just before the euthanasia. RESULTS In the group of matrix + peritoneum, the macroscopic examination showed a retraction of approximately 30% of the initial size of the patch. Microscopic examination showed: mucous ulceration, moderate fibrosis and a disorganized muscular layer. There was a high level of interstitial fibrosis. There was no signs of urothelial reepithelisation, although we observed many parts of glandular cystic cystitis and few parts of neovascularization, specially in the transition part. There were not traces of the matrix. In the group that we used only peritoneum, the retraction was approximately 80% of the initial size of the patch, and microscopic examination showed only mucous ulceration and fibrosis. CONCLUSIONS The use of a matrix of collagen and elastin covered with own parietal peritoneum in order to make a bladder augmentation in female pigs is better than using only a flap of parietal peritoneum. However, both procedures involve a moderate macroscopic retraction and fibrosis (higher in the peritoneum group). Only in the matrix group we observed an emerging development of a new-born tissue (neovascularization, mucose ulceration, glandular cystic cystitis). There were no significative changes in the cystometry in both groups before and after six weeks of the augmentation. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e478-e479 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Carlos Gasanz Serrano More articles by this author Carles Xavier Raventos Busquets More articles by this author Jordi Temprana Salvador More articles by this author Lucas Regis Placido More articles by this author Pol Servian Vives More articles by this author Ricardo Lopez del Campo More articles by this author Ignacio Arroyo Soto More articles by this author Enric Miret Alomar More articles by this author Merce Cuadras Soler More articles by this author Luis Castro Sader More articles by this author Cristian Konstantinidis Garay More articles by this author Marielle Esteves Coelho More articles by this author Carla Fonseca More articles by this author Ines de Torres More articles by this author Juan Morote Robles More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...