MP34-08 ETIOLOGY OF RECURRENT CYSTITIS BASED ON VAGINAL MICROBIOME: THINKING BEYOND THE BLADDER

Abstract

You have accessJournal of UrologyCME1 Apr 2023MP34-08 ETIOLOGY OF RECURRENT CYSTITIS BASED ON VAGINAL MICROBIOME: THINKING BEYOND THE BLADDER Takuya Sadahira, Takanori Sekito, Takehiro Iwata, Yuki Maruyama, and Motoo Araki Takuya SadahiraTakuya Sadahira More articles by this author , Takanori SekitoTakanori Sekito More articles by this author , Takehiro IwataTakehiro Iwata More articles by this author , Yuki MaruyamaYuki Maruyama More articles by this author , and Motoo ArakiMotoo Araki More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003268.08AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: We aimed to determine the etiology of recurrent cystitis from vaginal microbiome and to evaluate the importance of the presence of Lactobacillus in the vagina. METHODS: Vaginal samples were obtained from postmenopausal women classified into four groups: healthy controls (“Healthy”) group, patients with uncomplicated cystitis (“Uncomplicated Cystitis”) group, patients with recurrent cystitis (“RC”) group, and patients with recurrent cystitis prevented by administration of Lactobacillus crispatus-containing vaginal suppositories (“Prevention”) group. 16S rRNA gene sequencing data from vaginal microbiome was analyzed among four groups by principal coordinate analysis. RESULTS: A total of 129 vaginal samples from 39 women were studied. The vaginal bacterial communities could be divided into three clusters (A, B, C), based on differences in relative abundances of bacterial taxa among samples (Figure 1A). Among the four groups of postmenopausal women, 39/39 (100%) samples from "Healthy" group were in Cluster A and B, 24/24 (100%) samples from "Uncomplicated Cystitis" group were in Cluster A and B, 3/4 (75%%) samples from "RC" group were in Cluster C, and 61/63 (97%) samples from "Prevention" group were in Cluster B and C. In principal coordinate analysis, the plots in the “Uncomplicated cystitis” group was similar to those of "Healthy" group (Figure 1A). In addition, principal coordinate analysis indicated a large separation between the plots in the “RC” group and "Uncomplicated cystitis" group. Among four patients in "RC" group, the median (range) relative abundance of Lactobacillus was not detected before administration of Lactobacillus crispatus-containing vaginal suppositories, while Lactobacillus was 18% (12%-25%) during administration on average reducing the onset of cystitis per year (7.8 vs 2.5, p=0.0061) (Figure 1B). CONCLUSIONS: The vaginal microbiome of recurrent cystitis is essentially different from that of uncomplicated cystitis. Moreover, lack of Lactobacillus in postmenopausal vaginal microbiome is associated with the development of recurrent cystitis. Vaginal administration of Lactobacillus prevents recurrent cystitis by improving vaginal dysbiosis. Source of Funding: none © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e462 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Takuya Sadahira More articles by this author Takanori Sekito More articles by this author Takehiro Iwata More articles by this author Yuki Maruyama More articles by this author Motoo Araki More articles by this author Expand All Advertisement PDF downloadLoading ...