MP30-02 EFFECTS OF OMEGA-3 FATTY ACIDS IN A RAT MODEL OF ATHEROSCLEROSIS-INDUCED CHRONIC BLADDER ISCHEMIA

Abstract

You have accessJournal of UrologyBladder & Urethra: Anatomy, Physiology & Pharmacology II1 Apr 2016MP30-02 EFFECTS OF OMEGA-3 FATTY ACIDS IN A RAT MODEL OF ATHEROSCLEROSIS-INDUCED CHRONIC BLADDER ISCHEMIA Ji Yun Chae, Hyoung Kook Jeong, Jong Jin Park, Jong Wook Kim, Mi Mi Oh, Jae Hyun Bae, Seok Ho Kang, Hong Seok Park, Du Geon Moon, Jun Cheon, Je Jong Kim, and Jeong Gu Lee Ji Yun ChaeJi Yun Chae More articles by this author , Hyoung Kook JeongHyoung Kook Jeong More articles by this author , Jong Jin ParkJong Jin Park More articles by this author , Jong Wook KimJong Wook Kim More articles by this author , Mi Mi OhMi Mi Oh More articles by this author , Jae Hyun BaeJae Hyun Bae More articles by this author , Seok Ho KangSeok Ho Kang More articles by this author , Hong Seok ParkHong Seok Park More articles by this author , Du Geon MoonDu Geon Moon More articles by this author , Jun CheonJun Cheon More articles by this author , Je Jong KimJe Jong Kim More articles by this author , and Jeong Gu LeeJeong Gu Lee More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.1234AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Aging-related bladder dysfunction and associated lower urinary tract symptoms (LUTS) have been well documented and seem to be multifactorial. One of the factors is chronic ischemia of the bladder detrusor, resulting from atherosclerotic changes in blood vessels. The aim of this study was to evaluate the potential protective effects of omega-3 fatty acids in a rat model of atherosclerosis- induced CBI. METHODS Total 18 of 16-week old male rats were divided into three groups. The CBI group underwent endothelial injury of the iliac arteries and received 2% cholesterol diet. The omega-3 fatty acids group underwent endothelial injury also and received 2% cholesterol diet, with 2 weeks of DHA + EPA (200mg/kg/day) administration. The control group underwent sham operation and received a regular diet. After 8 weeks, in vivo cystometry and in vitro detrusor muscle strip study were performed. The plasma and bladder tissue were processed for immunohistochemical staining of oxidative stress markers. Histological examination of the bladder and iliac arteries was also done. RESULTS In cystometry, omega-3 fatty acids group showed decreased micturition frequency with prolonged intercontraction interval, increased bladder capacity compared to untreated CBI group. In the muscle strip study, omega-3 fatty acids group showed increased contractile responses compared to untreated CBI group. Plasma and bladder tissue of CBI group showed higher malondialdehyde (MDA) than in other groups. In histological examination, omega-3 fatty acids group showed lesser submucosal fibrosis of the bladder wall and also lesser degenerative change of blood vessel wall than in CBI group. CONCLUSIONS Atherosclerosis-induced CBI is associated with detrusor overactivity in cystometric study, impaired smooth muscle contractility in muscle strip study, and oxidative stress in MDA assay. Our study suggests that omega-3 fatty acids supplementation provides bladder protection against CBI on rat urinary bladder and reduces the MDA accumulation in bladder tissue caused by chronic ischemia and reperfusion. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e411-e412 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Ji Yun Chae More articles by this author Hyoung Kook Jeong More articles by this author Jong Jin Park More articles by this author Jong Wook Kim More articles by this author Mi Mi Oh More articles by this author Jae Hyun Bae More articles by this author Seok Ho Kang More articles by this author Hong Seok Park More articles by this author Du Geon Moon More articles by this author Jun Cheon More articles by this author Je Jong Kim More articles by this author Jeong Gu Lee More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...