MP29-03 SUCCESSFUL LAPAROSCOPIC ALLOTRANSPLANTATION OF EMBRYONIC KIDNEYS ENHANCED BY ADIPOSE-DERIVED STEM CELLS SUSPENSION

Abstract

You have accessJournal of UrologyTransplantation & Vascular Surgery: Renal Transplantation & Vascular Surgery I1 Apr 2016MP29-03 SUCCESSFUL LAPAROSCOPIC ALLOTRANSPLANTATION OF EMBRYONIC KIDNEYS ENHANCED BY ADIPOSE-DERIVED STEM CELLS SUSPENSION César Vera-Donoso, Ximo García-Domínguez, Victoria Moreno-Manzano, Luís García-Valero, José Salvador Vicente-Antón, and Francisco Marco-Jiménez César Vera-DonosoCésar Vera-Donoso More articles by this author , Ximo García-DomínguezXimo García-Domínguez More articles by this author , Victoria Moreno-ManzanoVictoria Moreno-Manzano More articles by this author , Luís García-ValeroLuís García-Valero More articles by this author , José Salvador Vicente-AntónJosé Salvador Vicente-Antón More articles by this author , and Francisco Marco-JiménezFrancisco Marco-Jiménez More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.1087AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The use of xenotransplants has been considered for years as a possible solution to the organ shortage, but the risks of xenograft loss rejection and zoonoses have limited the clinical application of this kind of treatments. There have been several recent attempts to generate, de novo, a functional whole kidney from embryonic kidney (metanephroi) avoided rejection from non-immunosuppressed hosts. We recently generated a functional kidney de novo using a minimally invasive laparoscopic procedure to trasplant metanephroi. Allowing the kidney to grow large is another important issue; therefore, we evaluated the addition of adipose-derived stromal vascular fraction cells (SVF) or adipose derived stem cells (ASC) during trasplantation METHODS Briefly, biopsy of subcutaneous adipose tissue was collected from adult male rabbits. Manually cut into small pieces, the fat was then digested overnight, washed and centrifuge several times to constituted the SVF. For ASC amplification, the SVF was then resuspended in growth medium and kept under standard cell culture conditions for at least one week. We transplanted laparoscopically metanephroi from rabbit embryos in 3 groups: a) combined treatment with 105 cells of either mononuclear cells from the SVF or b) combined with ASC and c) control suspended in 5 µl of growth medium to test in vivo host integration and function. Then we transplant every metanephros by a laparoscopic procedure and documented the growth (weight, morphologically and histomorphometric of glomeruli) and the renin and erythropoietin transcript expression by qRT-PCR RESULTS Four weeks after transplantation, kidney graft explants were recovered and examined. In total, 3/6 SVF metanephroi, 4/6 ASC metanephroi and 3/6 control metanephroi were successfully grown. However, in the ASC group, the organ weights increased significantly (0.5±0.06 g vs 0.2±0.08 g and 0.3±0.08 g for ASC vs SVF and control group, respectively p < 0.05). In all of groups, kidney graft explants developed mature glomeruli whose histomorphometry analysis showed a similar area and perimeter of renal corpuscle and glomerulus and glomerular tuft cellularity. In addition, the expression profile of renin and erythropoietin transcript, and consistent with the expression in host kidneys CONCLUSIONS Taken together, our results demonstrated that embryonic kidney in combined treatment with adipose derived stem cells, enable the generation of kidney graft explants with higher weights after laparoscopic transplantation into syngeneic non-immunosuppressed host © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e380-e381 Advertisement Copyright & Permissions© 2016MetricsAuthor Information César Vera-Donoso More articles by this author Ximo García-Domínguez More articles by this author Victoria Moreno-Manzano More articles by this author Luís García-Valero More articles by this author José Salvador Vicente-Antón More articles by this author Francisco Marco-Jiménez More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...