MP28-09 A NOVEL MODEL OF PELVIC ORGAN ISCHEMIA: PELVIC FLOOR CONTRACTILE FUNCTION IS MORE RESISTANT TO ISCHEMIA AND POST-ISCHEMIA REPERFUSION THAN BLADDER DETRUSOR MUSCLE

Abstract

You have accessJournal of UrologyBladder & Urethra: Anatomy, Physiology & Pharmacology I1 Apr 2016MP28-09 A NOVEL MODEL OF PELVIC ORGAN ISCHEMIA: PELVIC FLOOR CONTRACTILE FUNCTION IS MORE RESISTANT TO ISCHEMIA AND POST-ISCHEMIA REPERFUSION THAN BLADDER DETRUSOR MUSCLE Amy D. Dobberfuhl, Catherine Schuler, Robert E. Leggett, Elise J.B. De, and Robert M. Levin Amy D. DobberfuhlAmy D. Dobberfuhl More articles by this author , Catherine SchulerCatherine Schuler More articles by this author , Robert E. LeggettRobert E. Leggett More articles by this author , Elise J.B. DeElise J.B. De More articles by this author , and Robert M. LevinRobert M. Levin More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.1059AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Weakness of the pelvic floor musculature (PFM) is well described in stress urinary incontinence and pelvic organ prolapse. Periods of low blood flow, hypoxia and ischemia have been implicated as causes of pelvic floor and bladder dysfunction. We explored the contractile characteristics of PFM and bladder detrusor muscle (BDM) in a novel model of in-vitro ischemia-reperfusion. METHODS Female adult virgin rabbits were selected for their well developed pelvic floor. Five animals were euthanized. BDM, pubococcygeus (PC) and coccygeous (CC) were isolated into 150 mg strips. Tissues were equilibrated with oxygenated Tyrodes containing glucose, stimulated with field stimulation, and then stimulated under ischemic conditions for 1 hour in nitrogenated Tyrodes without glucose. Tissues were then incubated in oxygenated Tyrodes for 2 hours and stimulated. RESULTS PFM required 10 times the stimulus duration to generate contractions under baseline conditions. Both maximal contractile response and rate of tension generation were significantly greater for BDM than either PC or CC. However, PC and CC were both significantly less sensitive to the effects of ischemia than BDM and continued to have stable contractile responses to field stimulation during the entire hour of ischemia (Figure 1). There was progressive decline in BDM contractile strength after 1 hour of ischemia. Following the ischemic period, PFM contractile recovery was significantly superior to BDM (Figure 2) and consistent with markers of oxidative stress. CONCLUSIONS PFM contractions and recovery were significantly slower than BDM, yet not as sensitive to either in-vitro ischemia or the effects of post-ischemia reperfusion. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e374 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Amy D. Dobberfuhl More articles by this author Catherine Schuler More articles by this author Robert E. Leggett More articles by this author Elise J.B. De More articles by this author Robert M. Levin More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...