MP27-09 OXYMATRINE IMPROVES ERECTILE FUNCTION THROUGH SUPPRESSION OF INFLAMMATION AND FIBROSIS IN DIABETIC RATS

Abstract

You have accessJournal of UrologyCME1 Apr 2023MP27-09 OXYMATRINE IMPROVES ERECTILE FUNCTION THROUGH SUPPRESSION OF INFLAMMATION AND FIBROSIS IN DIABETIC RATS Wenchao Xu, Hao Li, Taotao Sun, Jiaxin Wang, Tao Wang, and Jihong Liu Wenchao XuWenchao Xu More articles by this author , Hao LiHao Li More articles by this author , Taotao SunTaotao Sun More articles by this author , Jiaxin WangJiaxin Wang More articles by this author , Tao WangTao Wang More articles by this author , and Jihong LiuJihong Liu More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003255.09AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Diabetic mellitus-induced erectile dysfunction (DMED) responds poorly to phosphodiesterase type 5 inhibitors (PDE5i). Oxymatrine (OMT) has broad-spectrum anti-inflammatory and anti-fibrosis effects, but whether it improves the erectile function of diabetic populations through the above-mentioned mechanism is unclear. We intend to explore the role of OMT in the treatment of DMED. METHODS: A rat model of DMED was constructed by a single intraperitoneal injection of 1% streptozotocin solution. Thirty-two DMED rats were randomly divided into control group, DMED group, DMED + OMT-Low (50 mg/kg/day) group and DMED + OMT-High (100 mg/kg/day) group. Erectile function was evaluated by measuring intracavernosal pressure (ICP) and mean arterial pressure (MAP). The penile shafts were collected for subsequent molecular biological and histological analysis. RESULTS: ICP/MAP indicated that the erectile function of rats in the DMED model group was severely impaired, which was rescued in the DMED + OMT-Low and DMED + OMT-High groups. Rats in the DMED model group can highly express inflammatory indicators NF-kb, TNF-α and fibrosis-related proteins TGF-beta, CTGF, and Smad 2/3. The above-mentioned penile inflammation and fibrosis changes can be inhibited by OMT. Meanwhile, our results also showed that after OMT treatment, the NO/cGMP pathway was promoted and the RhoA/ROCK pathway was inhibited in the corpus cavernosum of DMED rats. CONCLUSIONS: OMT suppresses inflammation and fibrosis in the corpus cavernosum of diabetic rats to improve erectile function. OMT could improve penile erection by influencing endothelial and smooth muscle function. Our study provides new insight into the therapeutic effect of OMT and preclinical evidence for a potential treatment strategy for DMED. Source of Funding: This research was funded by grants from the National Natural Science Foundation of China (No. 82001536 and No.81873831) and the Fundamental Research Funds for the Central Universities (HUST: YCJJ202201021) © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e365 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Wenchao Xu More articles by this author Hao Li More articles by this author Taotao Sun More articles by this author Jiaxin Wang More articles by this author Tao Wang More articles by this author Jihong Liu More articles by this author Expand All Advertisement PDF downloadLoading ...