MP25-05 ENHANCED BLADDER PAIN SENSITIVITY DUE TO THE PROSTATE-TO-BLADDER AFFERENT CROSS SENSITIZATION IN A RAT MODEL OF PROSTATITIS

Abstract

You have accessJournal of UrologyInfections/Inflammation of the Genitourinary Tract: Prostate & Genitalia1 Apr 2015MP25-05 ENHANCED BLADDER PAIN SENSITIVITY DUE TO THE PROSTATE-TO-BLADDER AFFERENT CROSS SENSITIZATION IN A RAT MODEL OF PROSTATITIS Tsuyoshi Majima, Yasuhito Funahashi, Katsumi Kadekawa, Momokazu Gotoh, and Naoki Yoshimura Tsuyoshi MajimaTsuyoshi Majima More articles by this author , Yasuhito FunahashiYasuhito Funahashi More articles by this author , Katsumi KadekawaKatsumi Kadekawa More articles by this author , Momokazu GotohMomokazu Gotoh More articles by this author , and Naoki YoshimuraNaoki Yoshimura More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.1208AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES In chronic pelvic pain syndrome/chronic prostatitis (CPPS/CP), patients suffer from pelvic pain derived not only from the prostate, but also from the lower urinary tract (LUT). However, the mechanisms remain unclear; therefore, we investigated whether prostatic inflammation induced enhanced pain sensitivity in the LUT and bladder overactivity using a rat model of prostatitis. METHODS Adult Male SD rats were divided into two groups; control and prostatitis groups. (1) Formalin was injected into the prostate in the prostatitis group, while saline was injected in the control group. The rats were placed in metabolic cages to evaluate the voiding behavior 1 week after injection. Cystometry was also performed 1 week after injection to measure intercontraction intervals (ICI) of the voiding reflex. (2) Resiniferatoxin (RTx) was instilled into the bladder to evaluate nociceptive behavior such as licking behavior (urethral pain) and freezing behavior (bladder pain) at 1 week after injection. (3) Organ-specific afferent neurons were labelled by retrograde axonal transport of fluorescent dyes, Fast Blue (FB) or DiI, injected into the bladder wall or the prostate, respectively. Dye-labelled afferent neurons in S1 DRG were dissected using laser-capture microdissection method, and mRNA levels of TRP and ATP receptors were measured by real-time RT-PCR. RESULTS 1) In the metabolic cage study, the average micturition volume was decreased significantly in prostatitis rats (n=6) (0.46 ± 0.08 to 0.33 ± 0.07 mL, p = 0.005) without changes in control rats (n=6)(0.42 ± 0.03 to 0.41 ± 0.03 mL). In cystometry, the average ICI was significantly shorter in prostatitis rats (n=7) than in control (n=6) (791 ± 139 vs 1175 ± 146 sec, p = 0.026). (2) There were no significant differences in licking events between two groups (n=6, each group). However, the number of freezing events during 10-15mins after RTx administration into the bladder was significantly (p=0.01) increased in the prostatitis group than in the control group (20±1 and 11±2, respectively). (3) TRPV1, TRPA1 and P2X2 mRNA were increased in not only prostate-innervating DiI-labeled neurons, but also double-labeled neurons and FB-labeled bladder neurons from prostatitis rats compared to control rats CONCLUSIONS These results indicate that prostatic inflammation enhances pain sensitivity of the bladder and induces bladder overactivity in rats, as often seen in patients with CPPS/CP. Also, the prostate-to-bladder afferent cross-sensitization could be a mechanism underlying pelvic pain symptoms in CPPS/CP. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e286 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Tsuyoshi Majima More articles by this author Yasuhito Funahashi More articles by this author Katsumi Kadekawa More articles by this author Momokazu Gotoh More articles by this author Naoki Yoshimura More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...