MP25-04 POSSIBLE NEW URINE MARKERS FOR CALCIUM OXALATE “STONE FORMERS”: MACROPHAGE-RELATED CYTOKINES/CHEMOKINES DETECTED BY MULTIPLEX ANALYSIS

Abstract

You have accessJournal of UrologyStone Disease: Basic Research II1 Apr 2014MP25-04 POSSIBLE NEW URINE MARKERS FOR CALCIUM OXALATE “STONE FORMERS”: MACROPHAGE-RELATED CYTOKINES/CHEMOKINES DETECTED BY MULTIPLEX ANALYSIS Atsushi Okada, Takahiro Yasui, Kazumi Taguchi, Yasuhiro Fujii, Kazuhiro Niimi, Shuzo Hamamoto, Masahito Hirose, Ryosuke Ando, Yasue Kubota, Yasunori Itoh, Keiichi Tozawa, and Kenjiro Kohri Atsushi OkadaAtsushi Okada More articles by this author , Takahiro YasuiTakahiro Yasui More articles by this author , Kazumi TaguchiKazumi Taguchi More articles by this author , Yasuhiro FujiiYasuhiro Fujii More articles by this author , Kazuhiro NiimiKazuhiro Niimi More articles by this author , Shuzo HamamotoShuzo Hamamoto More articles by this author , Masahito HiroseMasahito Hirose More articles by this author , Ryosuke AndoRyosuke Ando More articles by this author , Yasue KubotaYasue Kubota More articles by this author , Yasunori ItohYasunori Itoh More articles by this author , Keiichi TozawaKeiichi Tozawa More articles by this author , and Kenjiro KohriKenjiro Kohri More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.306AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES In our previous series of basic studies, we showed that anti-inflammatory (M2) macrophages (Mϕ) might provide the main defense. However, the association between M2s and urinary stones has not been investigated in humans. This study aimed to identify M2-related urinary markers specific to calcium oxalate “stone formers” using multiplex analysis. METHODS The subjects were patients who consulted Nagoya City University Hospital: a control group (N = 56) with no history of urinary stones, first-time stone formers (N = 24), and recurrent stone formers (N = 42). No subject showed any apparent abnormality (hematuria, pyuria, proteinuria, or glucosuria) upon urinalysis; had any history of tumors, collagenosis, or immunologic abnormalities; or was taking any immunosuppressive drug. Urine for spot analysis was collected with each subject’s consent. Using 100 μl of urine, multiplex analysis was performed using the MagPix® system. We simultaneously measured the urinary concentrations of 19 proteins included in Human Cytokine/Chemokine Panel I, including 1) M2-related proteins, 2) proteins for which the mRNA expression level changed significantly in a microarray analysis of animal model, and 3) proteins previously shown to be associated with urinary stones. Each value is shown as the creatinine ratio. RESULTS Three of the 19 proteins included in the multiplex analysis differed significantly among the groups. Two of these were inflammatory chemokines: the level of growth-regulated protein (GRO/CXCL1) was significantly higher in the recurrent stone formers (2.13-fold over the control group, p = 0.0062) and that of monocyte chemoattractant protein-1 (MCP-1/CCL2) significantly higher in the first-time and recurrent stone formers (1.34- and 1.31-fold over the control group, p = 0.0274 and 0.0072, respectively). An anti-inflammatory cytokine involved in M2-Mϕ differentiation, interleukin-4 (IL-4), was present at significantly lower levels in the first-time and recurrent stone formers (0.20 and 0.68 times the control group value, p < 0.0001 and 0.0062, respectively). CONCLUSIONS Urinary excretion of inflammatory chemokines was significantly higher and that of the anti-inflammatory Mϕ-migration factor significantly lower in stone formers than in non-formers. The balance of inflammatory Mϕ activation and anti-inflammatory Mϕ suppression in the kidney may participate in kidney stone formation. In addition, these factors have potential for use as new urine markers for urinary stone risk. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e268 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Atsushi Okada More articles by this author Takahiro Yasui More articles by this author Kazumi Taguchi More articles by this author Yasuhiro Fujii More articles by this author Kazuhiro Niimi More articles by this author Shuzo Hamamoto More articles by this author Masahito Hirose More articles by this author Ryosuke Ando More articles by this author Yasue Kubota More articles by this author Yasunori Itoh More articles by this author Keiichi Tozawa More articles by this author Kenjiro Kohri More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...