MP25-02 THE NORMAL HEALTHY URINARY MICROBIOME IN THE MAPP NETWORK STUDY: RELATION TO GENDER, AGE AND MENOPAUSAL STATUS

Abstract

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Kidney & Bladder I (MP25)1 Sep 2021MP25-02 THE NORMAL HEALTHY URINARY MICROBIOME IN THE MAPP NETWORK STUDY: RELATION TO GENDER, AGE AND MENOPAUSAL STATUS J. Curtis Nickel, Alisa Stephens, A. Lenore Ackerman, Jennifer Anger, Henry LaI, and Garth D Ehrlich J. Curtis NickelJ. Curtis Nickel More articles by this author , Alisa StephensAlisa Stephens More articles by this author , A. Lenore AckermanA. Lenore Ackerman More articles by this author , Jennifer AngerJennifer Anger More articles by this author , Henry LaIHenry LaI More articles by this author , and Garth D EhrlichGarth D Ehrlich More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002022.02AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: To understand the role of the urinary microbiome in disease states and interpret non-culture-based diagnostic urine testing, we must have a better understanding of the microbiome in asymptomatic healthy individuals. We examined the impact of gender, age, and hormonal status on the healthy human urinary microbiome in asymptomatic control subjects enrolled in the multi-institution NIDDK Multidisciplinary Approach to the Study of Chronic Pelvic Pain Network (MAPP) study. METHODS: Asymptomatic healthy controls, recruited to be age- and sex-matched to patients in the Trans-MAPP Epidemiology and Phenotyping Study, provided midstream urine collection for PCR-electrospray ionization mass spectrometry identification of urinary microbiota. The microbiomes of male and female participants were described and analyzed for differences in composition and diversity at the species and genus level by sex, age, and, in females, by hormonal status. RESULTS: The total number of species detected was 66 with mean of 1.2 species (sd 1.1) per male (n=97; mean age =43) and 2.3 (sd 1.3) per female (n=110, mean age = 38) in asymptomatic healthy controls. Species and genera diversity analyses showed significantly greater richness and diversity in females. In regard to species (Figure), Bifidobacterium subtile, Lactobacillus crispatus, and Lactobacillus johnsonii were more predominant in females. The genera Bifidobacterium, Staphylococcus, Lactobacillus, and Corynebacterium were more predominant in females. While for males the most prevalent organisms included Staphylococcus and Propionibacterium, only Propionibacterium approached a significant difference between genders. No significant difference in the presence and/or diversity of micro-organisms with hormonal status could be observed. Sex-specific age trends, particularly diversity, were larger for females than males (Figure). CONCLUSIONS: The urinary microbiome of healthy asymptomatic subjects differed between genders and age in females, but not hormonal status. Gender differences may be attributable to the detection of urethral/vaginal organisms in females and prostate organisms in males. These findings will better allow us to interpret the results of microbiome reports in the midstream urine specimens of patients with urinary symptoms. Source of Funding: NIH/NIDDK © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e453-e454 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information J. Curtis Nickel More articles by this author Alisa Stephens More articles by this author A. Lenore Ackerman More articles by this author Jennifer Anger More articles by this author Henry LaI More articles by this author Garth D Ehrlich More articles by this author Expand All Advertisement Loading ...