Abstract

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Kidney & Bladder I1 Apr 2016MP24-12 MOLECULAR CHARACTERISTICS OF EXTENDED-SPECTRUM ?-LACTAMASE-PRODUCING ESCHERICHIA COLI ISOLATED URINARY TRACT INFECTION IN A UNIVERSITY TEACHING HOSPITAL Katsumi Shigemura, Kayo Osawa, Kazushi Tanaka, Yuzo Nakano, Toshiro Shirakawa, Soichi Arakawa, and Masato Fujisawa Katsumi ShigemuraKatsumi Shigemura More articles by this author , Kayo OsawaKayo Osawa More articles by this author , Kazushi TanakaKazushi Tanaka More articles by this author , Yuzo NakanoYuzo Nakano More articles by this author , Toshiro ShirakawaToshiro Shirakawa More articles by this author , Soichi ArakawaSoichi Arakawa More articles by this author , and Masato FujisawaMasato Fujisawa More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.768AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The prevalence of extended-spectrum β-lactamases (ESBLs) has been increasing worldwide. Recently, a pandemic clone of Escherichia coli O25:H4, sequence type 131 (ST131), producing ESBL-type CTX-M-15 has been reported as a major problem. In this study, we investigated the molecular characteristics of ESBL-producing E. coli isolates. METHODS ESBL-producing E. coli strains were isolated from urinary tract infection (UTI) patients in Kobe University Hospital for one year. Antibiotic susceptibilities were tested these strains using penicillin, cephem, carbapenem, monobactam, macrolide, aminoglycoside, tetracycline, quinolone and sulfa. We detected the ESBL blaCTX-M gene and nine virulence factor genes (papC, papEF, fimH, hlyA, iutA, sfa, eaeA, bfpA, and aggR) by PCR and DNA sequencing, plasmid replicon typing (IncF, IncFII, IncFIA, IncFIB, IncFIC, IncHII, IncHI2, IncI1-Ic, IncL/M, IncW, IncT, IncA/C, IncK, IncN, IncP, IncX, IncY, and IncB/O), phylogenetic grouping (A, B1, B2, and D), repetitive-sequence-based PCR (rep-PCR), and multilocus sequence typing. RESULTS All strains showed resistance to penicillin and cephems, and 68.1 % of strains shoed high resistance to levofloxacin. All strains were positive for blaCTX-M. The 30.6 % of strains in CTX-M-1 group included 12.5 % of CTX-M-15, 4.2 % in CTX-M-2 group, and the 65.3 % of strains in CTX-M-9 group. Rep-PCR was divided into 18 (a-r) groups and the most phylogenetic group was B2 group, detected in 68.1 % of strains. The CTX-M-15-producing E. coli O25:H4 ST131 was derived from phylogenetic group B2 and rep-PCR pattern d. The most prevalent virulence factor was fimH (100 %) and the most common replicon type was the IncF type (90.3 %). The CTX-M-9 group was significantly associated with the presence of papC and papEF [OR (95% CI) = 9.22 (1.32-64.7), p=0.025] or hlyA [OR (95% CI) = 5.57 (1.17-26.4), p=0.031]. CONCLUSIONS We confirmed that all ESBL-producing E. coli O25 strains isolated from UTI patients, including clone ST131-producing CTX-M-15, were derived from phylogenetic group B2, and this was consistent with rep-PCR analyses. CTX-M-15-producing E. coli O25:H4 ST131 with virulence factors, including adhesion factors, have emerged in Japan and it was found significant virulence factors with CTX-M-9 group. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e274 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Katsumi Shigemura More articles by this author Kayo Osawa More articles by this author Kazushi Tanaka More articles by this author Yuzo Nakano More articles by this author Toshiro Shirakawa More articles by this author Soichi Arakawa More articles by this author Masato Fujisawa More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...

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