MP24-18 DETRUSOR BIOENGINEERING USING COMPRESSED COLLAGEN, ADIPOSE-DERIVED STEM CELLS AND SMOOTH MUSCLE CELLS

Abstract

You have accessJournal of UrologyTrauma/Reconstruction/Diversion: Ureter (including Pyeloplasty) and Bladder Reconstruction (including fistula), Augmentation, Substitution, Diversion I1 Apr 2017MP24-18 DETRUSOR BIOENGINEERING USING COMPRESSED COLLAGEN, ADIPOSE-DERIVED STEM CELLS AND SMOOTH MUSCLE CELLS Jakub Smolar, Maya Horst, and Daniel Eberli Jakub SmolarJakub Smolar More articles by this author , Maya HorstMaya Horst More articles by this author , and Daniel EberliDaniel Eberli More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2017.02.3312AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Conditions impairing bladder function in children and adults often need urinary diversion or augmentation cystoplasty as when untreated they may cause severe bladder dysfunction and kidney failure. Currently, the gold standard therapy of end-stage bladder disease refractory to conservative management is enterocystoplasty, which despite providing functional improvement is associated with significant long-term complications. Therefore, there is a strong clinical need for alternative therapies for these reconstructive procedures. The aim of this study is to develop functional smooth muscle tissue for the detrusor muscle repair combining various cell types in hydrogel scaffolds. METHODS Rat bladder smooth muscle (SMC) and adipose-derived stem cells (ADSC) were isolated, expanded and characterized using flow cytometry. ADSC were pre-differentiated into SMC-like cells (pADSC). Cells were combined in ratios 1:1, 1:2 and 1:3 (SMC:pADSC) and embedded in compressed collagen (CC). After 1, 2 and 3 weeks, cells in CC scaffolds, direct and indirect 2D co-cultures were analyzed for viability, proliferation, morphology, SMC-marker expression and functionality. RESULTS Cell growth conditions have been optimized and cells have shown high viability and good proliferation in the CC scaffolds. Interconnected microtissues and cell layers have developed all over the CC already after 1 week of co-culture. At 1 and 2 week timepoints cells in CC showed strong expression of the SMC markers calponin, MyH11 and smoothelin. Direct cell co-culture resulted in significantly increased cellular proliferation. Microtissues consisted of a SMC-core surrounded by pADSC. Indirect co-culture resulted in an increased pADSC survival and ratio-dependent increase in SMC-proliferation. pADSC proliferation rate also improved, but remained unaffected by the cell ratio, with 1:1 showing the most consistent results. SMC-marker expression normalized between the different ratios after 2 weeks of co-culture and reached almost the SMC monoculture expression levels. The 1:1 co-culture contracted significantly better than the other ratios after 24h. CONCLUSIONS We have shown that a SMC–pADSC co-culture results in an improved cell survival, proliferation, microtissue and cell layer formation without any significant changes in phenotype and functionality. The combination of SMC and pADSC with CC may help to engineer functional detrusor muscle tissue by solving the major issues of tissue engineering, namely poor cell survival, proliferation, phenotype instability and functionality. © 2017FiguresReferencesRelatedDetails Volume 197Issue 4SApril 2017Page: e308-e309 Advertisement Copyright & Permissions© 2017MetricsAuthor Information Jakub Smolar More articles by this author Maya Horst More articles by this author Daniel Eberli More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...