MP23-19 FIBRINOGEN DEPOSITS ON URINARY CATHETERS IN A TIME-DEPENDENT MATTER AND CO-LOCALIZES WITH E. FAECALIS IN PATIENTS WITH POSITIVE E. FAECALIS URINE CULTURES

Abstract

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Kidney & Bladder II1 Apr 2017MP23-19 FIBRINOGEN DEPOSITS ON URINARY CATHETERS IN A TIME-DEPENDENT MATTER AND CO-LOCALIZES WITH E. FAECALIS IN PATIENTS WITH POSITIVE E. FAECALIS URINE CULTURES Tyler M. Bauman, Aaron M. Potretzke, Ana L. Flores-Mireles, Jennifer N. Walker, Alyssa M. Park, Henry L. Schreiber, Jerome S. Pinkner, Michael G. Caparon, Scott J. Hultgren, and Alana Desai Tyler M. BaumanTyler M. Bauman More articles by this author , Aaron M. PotretzkeAaron M. Potretzke More articles by this author , Ana L. Flores-MirelesAna L. Flores-Mireles More articles by this author , Jennifer N. WalkerJennifer N. Walker More articles by this author , Alyssa M. ParkAlyssa M. Park More articles by this author , Henry L. SchreiberHenry L. Schreiber More articles by this author , Jerome S. PinknerJerome S. Pinkner More articles by this author , Michael G. CaparonMichael G. Caparon More articles by this author , Scott J. HultgrenScott J. Hultgren More articles by this author , and Alana DesaiAlana Desai More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2017.02.747AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES There is mounting evidence that fibrinogen deposition on urinary catheters is a key step in the pathogenesis of catheter-associated urinary tract infection (CAUTI). The aim of this study was to investigate whether fibrinogen and Enterococcus faecalis co-localize on catheters acquired from patients with post-operative urine cultures positive for E. faecalis. METHODS Urinary catheters from a series of 50 patients undergoing elective urologic procedures were collected post-operatively and analyzed via immunofluorescence to detect deposited fibrinogen. Pearson correlation was performed to measure the correlation between fibrinogen deposition and dwell time. Additional catheters and urine cultures were collected at time of catheter removal. Catheters from patients with positive Enterococcus cultures were probed for fibrinogen and Enterococcus via immunofluorescence. RESULTS A total of 50 adult patients undergoing urinary catheterization as standard of care were prospectively identified at our institution. Fibrinogen concentration quantified as mg/catheter using a standard curve was highly correlated with catheter dwell time (r=0.63; p<0.0001) (Figure 1). E. faecalis was capable of binding to fibrinogen on these catheters ex vivo.Five additional catheters were obtained from patients with E. faecalis-positive post-operative urine cultures. Fibrinogen was present at all time points (18 hours, 1 day, 1 day, 8 days, 9 days) and co-localized with E. faecalis (Figure 2) in vivo. CONCLUSIONS We have previously shown that fibrinogen deposits on urinary catheters, and that E. faecalis is capable of binding to these catheters ex vivo. In this study, we demonstrate that E. faecalis co-localizes with fibrinogen in catheterized patients with urine cultures positive for E. faecalis. This data strengthens the clinical association of fibrinogen deposition with CAUTI and suggests that targeting the binding of E. faecalis with fibrinogen may help reduce the rate of Enterococcus CAUTI. © 2017FiguresReferencesRelatedDetails Volume 197Issue 4SApril 2017Page: e301 Advertisement Copyright & Permissions© 2017MetricsAuthor Information Tyler M. Bauman More articles by this author Aaron M. Potretzke More articles by this author Ana L. Flores-Mireles More articles by this author Jennifer N. Walker More articles by this author Alyssa M. Park More articles by this author Henry L. Schreiber More articles by this author Jerome S. Pinkner More articles by this author Michael G. Caparon More articles by this author Scott J. Hultgren More articles by this author Alana Desai More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...