MP21-08 SKELETAL MYOGENIC DIFFERENTIATION OF HUMAN URINE-DERIVED CELLS AS A POTENTIAL SOURCE OF CELL THERAPY FOR URETHRAL SPHINCTER MUSCLE DYSFUNCTION

Abstract

You have accessJournal of UrologyBladder and Urethra: Anatomy, Physiology and Pharmacology II1 Apr 2015MP21-08 SKELETAL MYOGENIC DIFFERENTIATION OF HUMAN URINE-DERIVED CELLS AS A POTENTIAL SOURCE OF CELL THERAPY FOR URETHRAL SPHINCTER MUSCLE DYSFUNCTION Wei Chen, M Xie, Bin Yang, S Bharadwaj, Song Li, Guihua Liu, Anthony Atala, and Yuanyuan Zhang Wei ChenWei Chen More articles by this author , M XieM Xie More articles by this author , Bin YangBin Yang More articles by this author , S BharadwajS Bharadwaj More articles by this author , Song LiSong Li More articles by this author , Guihua LiuGuihua Liu More articles by this author , Anthony AtalaAnthony Atala More articles by this author , and Yuanyuan ZhangYuanyuan Zhang More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.960AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Adult stem cells are regarded as possible cell therapy candidates for skeletal muscle regeneration in treatment of urethral sphincter dysfunction. However, invasive harvesting of those cells can cause potential harvest-site morbidity. The goal of this study was to assess whether human urine-derived stem cells (USCs), obtained through non-invasive procedures, can differentiate into skeletal muscle lineage cells, and potentially be used for skeletal muscle regeneration when treating urinary incontinence due to urethral sphincter dysfunction. METHODS USCs were harvested from six healthy individuals aged 25–55 years old. Expression profiles of cell-surface markers were assessed by flow cytometry. Growth factors and cytokines secreted from USCs and human bone marrow stroma cells (BMSC, as a control) culture supernatant were measure by specific ELISAs. To optimize the myogenic differentiation medium, we selected two from four different types of myogenic differentiation media to induce the USCs. Differentiated USCs were identified with myogenic markers by gene and protein expression. USCs were implanted into the tibialis anterior muscles of nude mice for 1 month. RESULTS Human USCs displayed surface markers with positive staining for CD24, CD29, CD44, CD73, CD90, CD105, CD117, CD133, CD146, SSEA-4 and STRO-1, and negative staining for CD14, CD31, CD34 and CD45. A series of growth factors and cytokines (such as IGF1, HGF, FGF, VEGF, and PDGF) were identified at higher levels in the culture supernatant of USCs than in BMSCs. After myogenic differentiation, morphology of USCs changed from ‘rice-grain’-like cells to spindle-shaped cells and then to myotube-like structures. The USCs expressed specific skeletal muscle transcripts and protein markers (myf5, myoD, myosin, and desmin) after being induced with different myogenic culture media. Implanted cells expressed skeletal muscle markers stably in vivo. CONCLUSIONS Our findings suggest that USCs can differentiate into a skeletal muscle lineage cells in vitro and after being implanted in vivo. Thus, they might be a potential source for cell injection therapy to induce urethral sphincter skeletal muscle regeneration and thereby treat urinary incontinence. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e235 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Wei Chen More articles by this author M Xie More articles by this author Bin Yang More articles by this author S Bharadwaj More articles by this author Song Li More articles by this author Guihua Liu More articles by this author Anthony Atala More articles by this author Yuanyuan Zhang More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...