MP21-09 CYSTOMETRIC EFFECTS OF ANDROGEN DEPRIVATION AND DAILY TADALAFIL ADMINISTRATION IN MICE WITH DETRUSOR OVERACTIVITY INDUCED BY NITRIC OXIDE CHRONIC DEFICIENCY

Abstract

You have accessJournal of UrologyBladder and Urethra: Anatomy, Physiology and Pharmacology II1 Apr 2015MP21-09 CYSTOMETRIC EFFECTS OF ANDROGEN DEPRIVATION AND DAILY TADALAFIL ADMINISTRATION IN MICE WITH DETRUSOR OVERACTIVITY INDUCED BY NITRIC OXIDE CHRONIC DEFICIENCY Raphael Farias Carvalho, Ricardo Reges, Patrícia Gaspar, Lucas Marinho, João Batista Cerqueira, and Lúcio Flávio Gonzaga-Silva Raphael Farias CarvalhoRaphael Farias Carvalho More articles by this author , Ricardo RegesRicardo Reges More articles by this author , Patrícia GasparPatrícia Gaspar More articles by this author , Lucas MarinhoLucas Marinho More articles by this author , João Batista CerqueiraJoão Batista Cerqueira More articles by this author , and Lúcio Flávio Gonzaga-SilvaLúcio Flávio Gonzaga-Silva More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.961AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Lower Urinary Tract Symptoms (LUTS) represents one of the most commonly complaints in male. Several voiding disorders can be involved in the pathogenesis of LUTS. Hipogonadism is a common and underdiagnosed disease in the aging male. This study aims to evaluate the cystometric effects of chronic tadalafil administration in castrated mice and the effects of testosterone replacement. METHODS A total of 42 mice were randomized to six groups. Group 1 (L-NAME): L-name (60mg/kg), which is an oxide-nitric sintethase inhibitor, was administrated in drinking water. Group 2 (DTAD): L-name + diluent of tadalafil (mannitol and xantane gum). Group 3 (TAD): L-name + daily tadalafil (4mg/kg). Group 4 (ORQ): L-name + orchiectomy. Group 5(ORQ+TAD): L-name + orchiectomy + tadalafil. Group 6 (TEST): L-name + ORQ + tadalafil + testosterone undecanoate SC single dose (100mg/kg). During cystometry, five parameters were measured: number of non-voiding contractions before first micturition (NVC) and micturition frequency (FM). Threshold pressure (TP), basal pressure (BP), and volume threshold (VT) were expressed as mmHg. Statistical analysis were held with GraphPad Prism®, using analysis of variance (ANOVA) and Tukey's tests. Level of significance was 5% (P<0.05). Mean and standard desviation (SD) were analysed. RESULTS TAD (1.28±1.89), ORQ(0.16±0.40), and TEST (1.14±1.21) Groups had lower number of NVC compared to L-name (5.42±4.11) Group. ORQ + TAD(1,5±1,64) and ORQ had similar similar NVC (p=0,0042). TAD (1.06±0.29) and ORQ (0.59±0.20) groups had lower FM compared L-name Group (2.16±0.50). ORQ + TAD (0.71±0.24) and ORQ had similar FM. TEST (2,97±0,45) had higher FM between groups (p<0,0001). TAD (27.57±7.41) and ORQ (27±5.51) Groups had lower TP compared to L-name (41.43±10.86). ORQ + TAD (32.17±7.27) and ORQ had similar TP. TEST (42.14±6.09) had higher TP compared to ORQ (27±5.51) and TAD Groups (p=0,0006). TAD (18.71±3.94) and ORQ (13±10.94) Groups had lower BP compared to L-name (31.14±4.56). ORQ(13±10.94) and ORQ+TAD (19.83±3.25) had similar BP. TEST(28±5.83) had higher BP compared to ORQ (p=0,0006). There were no statistical diferences in volume threshold between groups. There were no diferences between DTAD and L-name in any parameter. CONCLUSIONS Tadalafil and castration were equally effective reducing detrusor overactivity in mice with nitric oxide chronic deficiency. Co-treatment of testosterone with tadalafil to hipogonadic rats improves frequency of micturition, basal and threshold pressure. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e235-e236 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Raphael Farias Carvalho More articles by this author Ricardo Reges More articles by this author Patrícia Gaspar More articles by this author Lucas Marinho More articles by this author João Batista Cerqueira More articles by this author Lúcio Flávio Gonzaga-Silva More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...