MP21-03 INHIBIN B IS A PREDICTIVE FACTOR OF SPERM RECOVERY IN NON-OBSTRUCTIVE AZOOSPERMIA

Abstract

You have accessJournal of UrologyInfertility: Epidemiology & Evaluation I (MP21)1 Sep 2021MP21-03 INHIBIN B IS A PREDICTIVE FACTOR OF SPERM RECOVERY IN NON-OBSTRUCTIVE AZOOSPERMIA Isadora Badalotti-Teloken, Mariangela Badalotti, Guilherme Santiago, Bruna Santos, Bruna Araujo, Ana Karoline Rosa, Alvaro Petracco, and Claudio Teloken Isadora Badalotti-TelokenIsadora Badalotti-Teloken More articles by this author , Mariangela BadalottiMariangela Badalotti More articles by this author , Guilherme SantiagoGuilherme Santiago More articles by this author , Bruna SantosBruna Santos More articles by this author , Bruna AraujoBruna Araujo More articles by this author , Ana Karoline RosaAna Karoline Rosa More articles by this author , Alvaro PetraccoAlvaro Petracco More articles by this author , and Claudio TelokenClaudio Teloken More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002006.03AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Inhibin B is a glycoprotein produced by seminiferous tubules, predominantly by Sertoli cells, and has been proposed as a non-invasive marker of spermatogenesis. This study aimed to assess the potential of inhibin B as a spermatogenesis marker compared with follicle-stimulating hormone (FSH) and in non-obstructive azoospermic men, its association with testicular cyto-histology, and its potential to predict the presence of sperm at testicular sperm extraction (TESE) procedure. METHODS: Clinical and laboratory data of men treated at a Brazilian human reproduction center were reviewed. To evaluate the correlation between inhibin B and FSH, the patients were divided in groups: non-obstructive azoospermia (NOA), severe oligospermia (SO, sperm concentration <5.106/ml), and mild to moderate oligospermia (MO, sperm concentration 5-14.9.106/ml); fertile normozoospermic patients composed the control group (CG). In the NOA group the correlation between inhibin B and testicular cyto-histology (CE–complete spermatogenesis; MA–maturation arrest at level of spermatid; SC–only Sertoli cells, spermatocyte or spermatogonia) was evaluated. Testicular specimens were obtained in 3 different areas of each testi. In patients who underwent TESE, the presence of sperm was evaluated. The statistical analysis was carried out using Kruskal-Wallis, Spearman, and Mann-Whitney U test, multiple regression and ROC curve, considering p<0.05 significant. RESULTS: A total of 246 cases was eligible; NOA n=135 (mean age 36.9 yo), SO n=89 (mean age 36.7 yo), MO n=10 (mean age 37.2 yo) and CG n=11. The median level of inhibin B (pg/ml) was 36.8 in NOA, 74.0 in SO, 109.0 in MO and 196.0 in CG (p<0.0001). The correlation between inhibin B and FSH was significant (r=0,701; p<0.0001). In the cyto-histological comparison, the median value of inhibin B was 87.6 in CE, 42.7 in MA and 22.0 in SC (p<0.0001). Patients with inhibin B ≥88,0 have a 73.3% chance of having spermatozoa or spermatid in cyto-histology, and those with inhibin B ≤22.0 have a 76.3% chance of not having these cells (p<0.0001). In the TESE results, the median level of inhibin B was 80.0 in the group with sperm and 27.6 in the group with no sperm. Inhibin B ≥88,0 also has an 85% positive predictive value for having spermatozoa in TESE (p=0.047). CONCLUSIONS: This study shows a cutoff point for inhibin B ≥ 88.0 for a good prognosis of finding sperm in azoospermic individuals for assisted reproduction through TESE. Furthermore, an inhibin B ≤22 points to a bad prognosis, suggesting that the patients look further into infertility treatment alternatives, such as sperm bank. Source of Funding: None © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e346-e347 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information Isadora Badalotti-Teloken More articles by this author Mariangela Badalotti More articles by this author Guilherme Santiago More articles by this author Bruna Santos More articles by this author Bruna Araujo More articles by this author Ana Karoline Rosa More articles by this author Alvaro Petracco More articles by this author Claudio Teloken More articles by this author Expand All Advertisement Loading ...