MP21-01 HYDROGEN SULFIDE IS A POTENT RELAXING FACTOR IN THE RAT BLADDER AND PROSTATE

Abstract

You have accessJournal of UrologyBladder and Urethra: Anatomy, Physiology and Pharmacology II1 Apr 2015MP21-01 HYDROGEN SULFIDE IS A POTENT RELAXING FACTOR IN THE RAT BLADDER AND PROSTATE Takahiro Shimizu, Shogo Shimizu, Kumiko Nakamura, Masashi Honda, Keiji Inoue, and Motoaki Saito Takahiro ShimizuTakahiro Shimizu More articles by this author , Shogo ShimizuShogo Shimizu More articles by this author , Kumiko NakamuraKumiko Nakamura More articles by this author , Masashi HondaMasashi Honda More articles by this author , Keiji InoueKeiji Inoue More articles by this author , and Motoaki SaitoMotoaki Saito More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.953AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Hydrogen sulfide (H2S) is a third endogenous gasotransmitter besides NO and CO, and induces smooth muscle relaxation. In this study, we investigated pharmacological profiles of exogenous H2S-induced relaxation of bladder and prostate smooth muscle and expression levels of H2S synthesis enzymes, cystathionine β-synthase (CBS) and 3-mercaptopyruvate sulftransferase (MPST), in these tissues of the rat. METHODS Bladder dome and trigone (BL-D and BL-T), and ventral and dorsolateral prostate (PR-V and PR-D) were prepared from male Wistar rats (350–400 g). Relaxation effects of NaHS, an H2S donor, were evaluated by organ bath studies on acetylcholine (1x10−5 M)-induced contractions of bladder and on noradrenaline (1x10−5 M)- induced ones of prostate. Protein levels of CBS and MPST in each part were also investigated by Western blotting. RESULTS NaHS dose-dependently elicited relaxation on BL-D and BL-T pre-contracted by acetylcholine and on PR-V and PR-D pre-contracted by noradrenaline. There were no significant differences in values of EC50 and relaxation rate against the pre-contractions among these tissues. CBS was detected in PR-V and PR-D, and the expression levels in PR-D were higher than those in PR-V. In BL-D and BL-T, CBS was not detected. MPST was detected in all these tissues. Expression levels of MPST in PR-V were the highest and those in prostate were higher than those in bladder. CONCLUSIONS H2S induces relaxation of bladder and prostate smooth muscle. H2S synthesis enzymes are expressed in both bladder and prostate. These results suggest that H2S could function as an endogenous relaxation factor in both tissues. Endogenous H2S might be a new therapeutic target for lower urinary tract dysfunction such as overactive bladder and benign prostatic hyperplasia. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e232 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Takahiro Shimizu More articles by this author Shogo Shimizu More articles by this author Kumiko Nakamura More articles by this author Masashi Honda More articles by this author Keiji Inoue More articles by this author Motoaki Saito More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...