MP19-16 HUMAN ADIPOSE TISSUE DERIVED STEM CELLS (HADSCS) PREVENTS FIBROSIS AND BLADDER DYSFUNCTION IN A RAT MODEL OF URETHRAL STRICTURE

Abstract

You have accessJournal of UrologyStem Cell Research1 Apr 2015MP19-16 HUMAN ADIPOSE TISSUE DERIVED STEM CELLS (HADSCS) PREVENTS FIBROSIS AND BLADDER DYSFUNCTION IN A RAT MODEL OF URETHRAL STRICTURE Fabio Castiglione, Maarten Albersen, Andrea Russo, Trinity J. Bivalacqua, Francesco Montorsi, Steven Joniau, Dirk De Ridder, and Petter Hedlund Fabio CastiglioneFabio Castiglione More articles by this author , Maarten AlbersenMaarten Albersen More articles by this author , Andrea RussoAndrea Russo More articles by this author , Trinity J. BivalacquaTrinity J. Bivalacqua More articles by this author , Francesco MontorsiFrancesco Montorsi More articles by this author , Steven JoniauSteven Joniau More articles by this author , Dirk De RidderDirk De Ridder More articles by this author , and Petter HedlundPetter Hedlund More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.1008AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The cure rate of urethrotomy for urethral stricture (US) is lower than 10%. Reduction of high recurrence rates would improve patient comfort and a decrease healthcare costs. We aimed to evaluate if local injection of hADSCs could prevent fibrosis in a rat model of iatrogenic US. METHODS After ethical approval, partial damage of the spongious urethra of male rats was created with a hypodermic needle. Then, TGFβ1 (1μg) was injected into the site of urethral damage. Rats were divided into 3 groups: vehicle (n=7), hADSCs (n=7), and sham (no damage; no TGFβ1, n=7). Vehicle (100μl) or hADSCs (1 million in 100μl) was injected to the site of urethral damage one day later. After 4 weeks, rats were implanted with bladder catheters and underwent awake cystometry. Following euthanasia, penises and bladders were harvested for histology and western blot analysis (WB). RESULTS Vehicle rats exhibited higher (p<0.05) threshold pressure (TP: 23.95 ± 3.4cmH2O), flow pressure (FP: 36.32 ± 2.89 cmH2O) and basal pressure (BP: 14.27 ± 2.39 cmH2O) compared to the hADSCs group (TP: 14.07 ± 1.44; FP: 26.05 ± 3.19; BP: 9.65 ± 0.89 cmH2O) and sham group. Vehicle rats showed lower (p<0.05) threshold compliance (TC: 0.064±0.025 ml/cmH2O) and flow compliance (FC: 0.022 ± 0.010 ml/cmH2O) than hADSCs (TC: 0.23±0.03; FC: 0.061 ± 0.09 ml/cmH2O) and sham group. No differences in these endpoints were detected between ADCS and Sham groups. Maximum micturition pressure was higher in vehicle and hADSCs groups than in the sham rats (p<0.05). By WB, collagen III expression was higher in penises of vehicle rats (2.25±0.25) than in sham (1.49±0.16) and hADSCs groups (1.13±0.27) (p<0.05). No differences were noted in elastin expression between the three groups (p>0.05). Collagen I was more expressed in vehicle and hADSCs groups compared to sham group (p<0.05). Interestingly, the eNOS expression was higher in hADSCs group compared to the other groups (p<0.05). The WB results were corroborated by immunofluorescence analysis. Bladder-to-body weight ratio was higher in vehicle group compared to hADSCs and sham group (p<0.05). By WB, vehicle bladders showed more collagen III than the others groups (p<0.05). No differences were detected in collagen I or elastin expressions between the groups. CONCLUSIONS Local injection of hADSCs seems to prevent fibrosis and counteract urodynamic changes in a male rat model for US. Additional studies are required to understand the role of eNOS in this process. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e221 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Fabio Castiglione More articles by this author Maarten Albersen More articles by this author Andrea Russo More articles by this author Trinity J. Bivalacqua More articles by this author Francesco Montorsi More articles by this author Steven Joniau More articles by this author Dirk De Ridder More articles by this author Petter Hedlund More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...