MP19-12 BLADDER EXPRESSION OF INTERLEUKIN IL-17 ATTENUATES INFLAMMATION ASSOCIATED DEGRADATION OF MRNA CODING FOR INFLAMMATORY CHEMOKINES AND RECEPTORS

Abstract

You have accessJournal of UrologyBenign Prostatic Hyperplasia: Basic Research1 Apr 2014MP19-12 BLADDER EXPRESSION OF INTERLEUKIN IL-17 ATTENUATES INFLAMMATION ASSOCIATED DEGRADATION OF MRNA CODING FOR INFLAMMATORY CHEMOKINES AND RECEPTORS Pradeep Tyagi, Mahendra Kashyap, Zhou Wang, and Naoki Yoshimura Pradeep TyagiPradeep Tyagi More articles by this author , Mahendra KashyapMahendra Kashyap More articles by this author , Zhou WangZhou Wang More articles by this author , and Naoki YoshimuraNaoki Yoshimura More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.713AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The contribution of IL-17 to prostate inflammation remains a subject of intense interest. Steiner et al Prostate 2003,demonstrated that IL-17 is only expressed by inflamed prostate. Here, we studied the association of IL-17 expression with the mRNA and protein levels of inflammatory chemokines in the bladder and prostate harvested from a rat model of non bacterial prostatitis(NBP). METHODS NBP was induced in adult male Sprague Dawley rat by intraprostatic injection of either 5% formalin in saline or saline into the ventral lobes of prostate with 50μL in each lobe (n=6). On 7th day, whole prostate and bladder were weighed and processed for qPCR and MILLIPLEX rat chemokine assay for IL-17, MCP-1, MIP-1a, CXCL-1, CXCL-10 & NGF. RESULTS NBP was associated with significant elevation of chemokine proteins in both prostate and bladder, with relatively higher expression in prostate compared to bladder. IL-17 protein levels were 65.62±11.15pg/mg of protein in bladder of NBP rat and undetectable in control bladder. IL-17 expression in controls and NBP prostate differed slightly without significance. The normalized levels of mRNA coding for the measured chemokines and their cognate receptors were several fold elevated in bladder of NBP rat relative to control (Fig.). In contrast, the mRNA levels of chemokines in NBP prostate, when normalized to control prostate were 10 fold lower than bladder levels. NBP seems to be associated with amplified decay of mRNA coding for chemokines in the prostate, but not in bladder. Prostate and bladder also differed in terms of IL-17 expression, which is known to attenuate the mRNA decay. CONCLUSIONS Appraisal of protein and mRNA levels of chemokines in prostate and bladder tissue harvested from NBP rats imply differential mRNA decay in the two adjoining tissues of lower urinary tract. Significantly elevated IL-17 expression in bladder was associated with higher mRNA levels, whereas the lack of IL-17 elevation in NBP prostate was associated with lower normalized levels of mRNA coding for chemokines. IL-17 is known to protect against micro RNA-induced decay that target AU-rich elements in the 3'-untranslated region of mRNAs coding for cytokines. These findings support IL-17 as an important drug target to arrest BPH progression. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e193 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Pradeep Tyagi More articles by this author Mahendra Kashyap More articles by this author Zhou Wang More articles by this author Naoki Yoshimura More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...