MP19-07 AUTOFLUORESCENCE OF RANDALL′S PLAQUE PRECURSORS

Abstract

You have accessJournal of UrologyStone Disease: Basic Research & Pathophysiology II1 Apr 2017MP19-07 AUTOFLUORESCENCE OF RANDALL′S PLAQUE PRECURSORS Ling Chen, Ryan Hsi, Benjamin Sherer, Marshall Stoller, and Sunita Ho Ling ChenLing Chen More articles by this author , Ryan HsiRyan Hsi More articles by this author , Benjamin ShererBenjamin Sherer More articles by this author , Marshall StollerMarshall Stoller More articles by this author , and Sunita HoSunita Ho More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2017.02.3249AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Randall′s plaques at the renal papillary tips are known sites of stone propagation, yet the inciting events leading to Randall′s plaque are poorly understood. Noncollagneous proteins (NCPs) including osteopontin have been identified in the sites of biomineralization and may be critical in the early formation of Randall′s plaques. In contrast to traditional bright-field and polarized microscopy, fluorescence microscopy has the potential to localize and map these proteinaceous regions. This study was undertaken to localize osteogenic noncollagenous proteins in renal papilla as a potential nidus toward stone pathogenesis. METHODS Whole human renal papillae including tissue proximal to the fornix with grossly visible Randall′s plaque were harvested en bloc after nephrectomy. Papillary tissues were processed in a routine fashion and longitudinal histologic sections were characterized using bright field (BF), polarized light (PM), fluorescence (FM), and scanning electron microscopy (SEM) techniques (see Figure) before and after decalcification using EDTA solution. Immunolocalization of NCPs including osteopontin was performed via immunogold labeling of ultrathin sections. RESULTS Mineralized tubules (average diameter 18µm) revealed strong auto-fluorescence in blue, green, and red spectra. After decalcification of the same sections, birefringence visible under polarized light (PM) microscopy disappeared. However, the auto-fluorescence potentially associated with NCPs remained intact. Correlative light and electron microscopy (CLEM) and ultrastructural analyses revealed the mineralized tubular structure in renal papilla is rich in osteopontin (gold particles in Figure). CONCLUSIONS Localization of precursors to Randall′s plaque was detected by auto-fluorescence. NCPs appear to closely associate with tubules of diameter of 18 µm consistent with vascular elements in renal papilla, and may be intimately associated with stone formation. © 2017FiguresReferencesRelatedDetails Volume 197Issue 4SApril 2017Page: e231-e232 Advertisement Copyright & Permissions© 2017MetricsAuthor Information Ling Chen More articles by this author Ryan Hsi More articles by this author Benjamin Sherer More articles by this author Marshall Stoller More articles by this author Sunita Ho More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...