MP17-09 THROMBOSPONDIN-4 PROMOTES BLADDER CANCER CELL MIGRATION AND INVASION BY UPREGULATING MMP9 EXPRESSION

Abstract

You have accessJournal of UrologyBladder Cancer: Basic Research & Pathophysiology II (MP17)1 Apr 2020MP17-09 THROMBOSPONDIN-4 PROMOTES BLADDER CANCER CELL MIGRATION AND INVASION BY UPREGULATING MMP9 EXPRESSION Yi-Chia Lin*, Po-Chun Chen, Shan-Che Yang, Te-Fu Tsai, Hung-En Chen, Kuang-Yu Chou, and Thomas I. Hwang Yi-Chia Lin*Yi-Chia Lin* More articles by this author , Po-Chun ChenPo-Chun Chen More articles by this author , Shan-Che YangShan-Che Yang More articles by this author , Te-Fu TsaiTe-Fu Tsai More articles by this author , Hung-En ChenHung-En Chen More articles by this author , Kuang-Yu ChouKuang-Yu Chou More articles by this author , and Thomas I. HwangThomas I. Hwang More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000842.09AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Patients with non-muscle-invasive bladder cancer (NMIBC) are at risk of recurrence to progress into muscle-invasive bladder cancer (MIBC), and the prognosis of patients with MIBC is determined by the presence of metastasis. Thrombospondin-4 (THBS4/TSP4), a secreted matricellular glycoprotein participates cell-to-extracellular matrix attachment and regulates various physiological and pathological processes. However, little is known about the prognostic role of TSP4 and its role in bladder cancer progression. METHODS: The expression level of TSP4 and its prognostic value in bladder cancer (BC) specimens was evaluated in online database GEPIA. Moreover, the protein expression level of TSP4 in BC and normal tissue was investigated in the tumor tissue array by the immunohistochemistry. The pro-invasive role of TSP4 in BC was examined by in vitro cell migration and invasion assay. Finally, the molecular mechanism of TSP4 on BC metastasis was defined by using Western blot and QPCR analyses. RESULTS: Based on the results of meta-analysis in GEPIA database and immunohistochemistry, we found that expression level of TSP4 was in accordance with the progression of BC, especially in MIBC. Interestingly, its expression is positively correlated with the matrix metalloproteinase-9 (MMP-9) expression, which are two well-characterized invasive markers in BC. Incubation with recombinant TSP4 dramatically promoted BC cell migration, invasion and MMP-9 expression. CONCLUSIONS: Conclusively, our present study first describes the prognostic role of TSP4 in BC and defines its pro-invasive role in BC via promoting MMP-9 expression. TSP-2 may represent a novel promising new biomarker and therapeutic target for treating BC. Source of Funding: none © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e229-e229 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Yi-Chia Lin* More articles by this author Po-Chun Chen More articles by this author Shan-Che Yang More articles by this author Te-Fu Tsai More articles by this author Hung-En Chen More articles by this author Kuang-Yu Chou More articles by this author Thomas I. Hwang More articles by this author Expand All Advertisement PDF downloadLoading ...