MP17-20 CHARACTERIZATION OF FUNCTIONAL BLADDER REGENERATION IN MICE

Abstract

You have accessJournal of UrologyBladder & Urethra: Anatomy, Physiology & Pharmacology1 Apr 2014MP17-20 CHARACTERIZATION OF FUNCTIONAL BLADDER REGENERATION IN MICE Mona Zarifpour, Jason Sandberg, Mehran Abolbashari, Manasi Vadhavkar, Sneha Kelkar, Aaron Mohs, Frank Marini, Karl−Erik Andersson, and George Christ Mona ZarifpourMona Zarifpour More articles by this author , Jason SandbergJason Sandberg More articles by this author , Mehran AbolbashariMehran Abolbashari More articles by this author , Manasi VadhavkarManasi Vadhavkar More articles by this author , Sneha KelkarSneha Kelkar More articles by this author , Aaron MohsAaron Mohs More articles by this author , Frank MariniFrank Marini More articles by this author , Karl−Erik AnderssonKarl−Erik Andersson More articles by this author , and George ChristGeorge Christ More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.553AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Previous studies have established that subtotal cystectomy (removal of 60-70% of the bladder) provides a valid and unique model of mammalian organ regeneration (1,2). The goal of this study was to characterize this phenomenon in mice, a species that can be genetically engineered to investigate a diverse array of molecular alterations of functional bladder regeneration, including specific cell−based fluorescence imaging technologies. METHODS Female C57 black mice underwent STC, and at 4 and 12 weeks post−STC, bladder regeneration was assessed via cystometry and ex vivo pharmacologic organ bath studies. Histology was also performed to measure bladder wall thickness and the composition/architecture of the bladder wall. RESULTS We observed a time−dependent increase in bladder capacity following STC, such that 12 weeks post−STC, the sizes of regenerated bladders and micturition volumes were indistinguishable from those of age−matched controls (Fig. 1A, C). Bladders emptied completely at all time points studied (i.e., no increases in residual volume), consistent with functional bladder regeneration. There were no significant differences in bladder wall thickness (Fig. 1B) from controls, or in the percentage of smooth muscle in the detrusor layer (not shown). Contraction to pharmacological activation and electrical field stimulation were present in isolated tissue strips from regenerating bladders but were lower than controls at all time points. Initial cell and tissue transplantation studies using fluorescently labeled mice (i.e., transfer of green fluorescence protein bone marrow into red fluorescent protein animals) revealed a participation and contribution of bone marrow derived populations toward functional bladder regeneration. CONCLUSIONS This study extends our investigation of mammalian bladder regeneration from rats to mice. Establishing a validated model for the study of de novo organ regeneration in mice will allow for further characterization of this phenomenon through detailed investigation of its molecular and cellular basis. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e143 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Mona Zarifpour More articles by this author Jason Sandberg More articles by this author Mehran Abolbashari More articles by this author Manasi Vadhavkar More articles by this author Sneha Kelkar More articles by this author Aaron Mohs More articles by this author Frank Marini More articles by this author Karl−Erik Andersson More articles by this author George Christ More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...