MP17-18 BIDIRECTIONAL COMMUNICATION BETWEEN AFFERENT NEURONS AND UROTHELIAL CELLS IN THE MOUSE URINARY BLADDER

Abstract

You have accessJournal of UrologyBladder & Urethra: Anatomy, Physiology & Pharmacology1 Apr 2014MP17-18 BIDIRECTIONAL COMMUNICATION BETWEEN AFFERENT NEURONS AND UROTHELIAL CELLS IN THE MOUSE URINARY BLADDER Youko Ikeda, Irina Zabbarova, and Anthony Kanai Youko IkedaYouko Ikeda More articles by this author , Irina ZabbarovaIrina Zabbarova More articles by this author , and Anthony KanaiAnthony Kanai More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.551AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES It has been widely hypothesized that urothelial cells communicate unidirectionally with afferent terminals to signal changes in the bladder lumen milieu. However, direct interactions between afferents and urothelial cells have not been clearly demonstrated. Our aim was to examine these interactions using pseudorabies virus (PRV) with the genetically-encoded Ca2+ probe, GCaMP to localize its fluorescence in the mouse urinary bladder. METHODS For dorsal root ganglia (DRG)-PRV injections, adult female C57Bl/10 mice were anesthetized and a hemilaminectomy performed at the L5-L6 vertebrae level to expose L6-S1 DRG. 2 μl of PVR468-GCaMP3 (106 pfu/ml) was slowly injected into each DRG using a 1.0 mm glass micropipette with a 10 μm beveled tip. The muscle and skin were sutured, animals allowed to recover and used for experiments after 2-3 days. For sham controls, sterile saline was injected in place of viral vector. In additional experiments, 10 μl of PRV was injected into the wall of the descending colon or tail muscle. Isolated DRG or whole bladder sheets were fixed, sectioned and examined for GCaMP fluorescence. RESULTS Following DRG-PRV injections GCaMP labeling was found in afferent nerves in the detrusor (DM) and within the urothelium (UT), but not the lamina propria (LP) (figure 1A). Labeling in the UT appeared punctate or contained inside vesicles, suggesting there may be urothelial uptake of GCaMP or PRV from afferents. In colon and tail injections, a similar GCaMP expression pattern was found compared to DRG injections (B and C). Colon/tail injected animals also had dense expression in L6/S1 DRG, demonstrating that these structures have shared afferent innervation with the bladder (D and E). Fluorescence was not detected in the DRG or bladders of sham control animals (not shown). CONCLUSIONS The established concept has been that urothelial cells signal to suburothelial afferents to ‘sense’ changes within the bladder lumen milieu. We have demonstrated that communication between afferents and urothelial cells is bidirectional, where afferents can also signal to urothelial cells. Changes in the dynamics of urothelial-afferent communication may underlie sensory disorders of the lower urinary tract. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e142 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Youko Ikeda More articles by this author Irina Zabbarova More articles by this author Anthony Kanai More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...