MP15-11 EXTRACORPOREAL SHOCKWAVE THERAPY ALLEVIATES INFLAMMATORY PAIN BY DOWN-REGULATING NLRP3 INFLAMMASOME IN EXPERIMENTAL CHRONIC PROSTATITIS AND CHRONIC PELVIC PAIN SYNDROME

Abstract

You have accessJournal of UrologyCME1 Apr 2023MP15-11 EXTRACORPOREAL SHOCKWAVE THERAPY ALLEVIATES INFLAMMATORY PAIN BY DOWN-REGULATING NLRP3 INFLAMMASOME IN EXPERIMENTAL CHRONIC PROSTATITIS AND CHRONIC PELVIC PAIN SYNDROME Woong Jin Bae, Dongho Shin, Jun Jie Piao, Soomin Kim, Yong Sun Choi, Bong Hee Park, Hyun Jin Jung, Samuel Sorkhi, Saager Chawla, Hyuk Jin Cho, Sung-Hoo Hong, Sae Woong Kim, and Mahadevan Raj Rajasekaran Woong Jin BaeWoong Jin Bae More articles by this author , Dongho ShinDongho Shin More articles by this author , Jun Jie PiaoJun Jie Piao More articles by this author , Soomin KimSoomin Kim More articles by this author , Yong Sun ChoiYong Sun Choi More articles by this author , Bong Hee ParkBong Hee Park More articles by this author , Hyun Jin JungHyun Jin Jung More articles by this author , Samuel SorkhiSamuel Sorkhi More articles by this author , Saager ChawlaSaager Chawla More articles by this author , Hyuk Jin ChoHyuk Jin Cho More articles by this author , Sung-Hoo HongSung-Hoo Hong More articles by this author , Sae Woong KimSae Woong Kim More articles by this author , and Mahadevan Raj RajasekaranMahadevan Raj Rajasekaran More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003235.11AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: To evaluate the anti-inflammatory and antioxidative effects of extracorporeal shockwave therapy (ESWT) on prostatitis using in-vitro as well as in-vivo experimental models and explore the mechanism. METHODS: RWPE-1 cells randomly divided into 5 groups: 1) RWPE-1 group (normal control), 2) LPS group (lipopolysaccharide inducing inflammation), 3) 0.1ESWT group (treated by 0.1 mJ/mm2 ESWT), 4) 0.2ESWT group (treated by 0.2 mJ/mm2 ESWT) and 5) 0.3ESWT group (treated by 0.3 mJ/mm2 ESWT). After ESWT administered, cells and supernatant were collected for ELISA and western blot. In vivo, Sprague-Dawley male rats (n=36) were randomly divided into 3 groups: 1) normal group, 2) prostatitis group, and 3) ESWT group (n=12 for each). Prostatitis was induced by 17 beta-estradiol and dihydrotestosterone (DHT) administration. An ESWT electromagnetic medical device, IMPO88 (Huons Meditech Co., Ltd, Korea) was used in this experiment. Four weeks after ESWT, pain index was assessed for all groups and prostate tissues were collected for immunohistochemistry, immunofluorescence, apoptosis analysis and Western blot. RESULTS: The optimal energy flux density of ESWT was 0.2 mJ/mm2 in vitro. ESWT ameliorated discomfort in rats with prostatitis. Inflammation was improved by ESWT in rats with prostatitis (p<0.05). Compared to normal rats, overexpressed NLRP3 inflammasomes triggered apoptosis which was improved by ESWT in prostatitis (p<0.05). TLR4-NFκB pathway was overactive with prostatitis, compared to normal and ESWT group (p<0.05). BAX/BAK pathway was inhibited by ESWT in prostatitis rats (p<0.05). CONCLUSIONS: ESWT improved CP/CPPS by reducing NLRP3 inflammasome and ameliorated apoptosis via inhibiting BAX/BAK pathway in a rat model. TLR4 might be the key protein bonding NLRP3 inflammasome and BAX/BAK pathway. ESWT might be a potential and promising approach for the treatment for CP/CPPS. Source of Funding: none © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e196 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Woong Jin Bae More articles by this author Dongho Shin More articles by this author Jun Jie Piao More articles by this author Soomin Kim More articles by this author Yong Sun Choi More articles by this author Bong Hee Park More articles by this author Hyun Jin Jung More articles by this author Samuel Sorkhi More articles by this author Saager Chawla More articles by this author Hyuk Jin Cho More articles by this author Sung-Hoo Hong More articles by this author Sae Woong Kim More articles by this author Mahadevan Raj Rajasekaran More articles by this author Expand All Advertisement PDF downloadLoading ...