MP15-08 CANNABINOIDS REDUCE CHRONIC PROSTATITIS AND CHRONIC PELVIC PAIN SYNDROME BY MODULATING TLR-4/NF-KB SIGNALING PATHWAY

Abstract

You have accessJournal of UrologyCME1 Apr 2023MP15-08 CANNABINOIDS REDUCE CHRONIC PROSTATITIS AND CHRONIC PELVIC PAIN SYNDROME BY MODULATING TLR-4/NF-KB SIGNALING PATHWAY Dongho Shin, Jun Jie Piao, Soomin Kim, Woong Jin Bae, Mahadevan Raj Rajasekaran, and Sae Woong Kim Dongho ShinDongho Shin More articles by this author , Jun Jie PiaoJun Jie Piao More articles by this author , Soomin KimSoomin Kim More articles by this author , Woong Jin BaeWoong Jin Bae More articles by this author , Mahadevan Raj RajasekaranMahadevan Raj Rajasekaran More articles by this author , and Sae Woong KimSae Woong Kim More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003235.08AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: The purpose of this study was to assess the effect and investigate the mechanism of cannabinoids on LPS-induced inflammation in RWPE-1 cells and dihydrotestosterone-induced prostatitis rat model. METHODS: RWPE-1 cells were randomly divided into five groups: (a) RWPE-1 group (normal control), (b) LPS group (lipopolysaccharide-induced inflammation) and (c) CBD group (LPS-induced RWPE-1 treated by Cannabidiol), (d) CBC group (LPS-induced RWPE-1 treated by Cannabichromene), (e) CBG group (LPS-induced RWPE-1 treated by Cannabigerol). Also prostatitis rat models were diveided into two groups: (f) CBD treated group, (g) placebo group. After administration of cannabinoids therapy, cells were collected for immunofluorescence and cells were taken from the supernatant for Western blot analysis. RESULTS: Three cannabinoids improved cellular inflammation in RWPE-1 by attenuating inflammation (p<0.01). Also, CBD improved Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) in rats when evaluated by von frey filaments exam. Cannabinoids reduced cyclooxygenase 2 (COX-2) expression by inhibiting the TLR4-NFκB pathway compared with the LPS group with in vitro prostatitis (p<0.05). TRAF2 mediates the ERK1/2-COX2 pathway. CONCLUSIONS: Cannabinoids ameliorate CP/CPPS and reduce inflammation by degrading COX-2 in the microenvironment through the TLR4-NFκB inhibitory pathway. thus suggesting that cannabinoids may be a potential and promising approach for the treatment of CP/CPPS. Source of Funding: none © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e194 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Dongho Shin More articles by this author Jun Jie Piao More articles by this author Soomin Kim More articles by this author Woong Jin Bae More articles by this author Mahadevan Raj Rajasekaran More articles by this author Sae Woong Kim More articles by this author Expand All Advertisement PDF downloadLoading ...