MP15-01 A COMMENSAL BACTERIAL STRAIN PREVENTS AND EFFECTIVELY AMELIORATES CHRONIC PELVIC PAIN

Abstract

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Prostate & Genitalia I1 Apr 2018MP15-01 A COMMENSAL BACTERIAL STRAIN PREVENTS AND EFFECTIVELY AMELIORATES CHRONIC PELVIC PAIN Stephen Murphy, Anthony Schaeffer, and Praveen Thumbikat Stephen MurphyStephen Murphy More articles by this author , Anthony SchaefferAnthony Schaeffer More articles by this author , and Praveen ThumbikatPraveen Thumbikat More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.514AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Chronic pelvic pain syndrome (CPPS) is a common disorder with limited effective treatments due to its unknown etiology. Previous studies from our laboratory have demonstrated that intra-urethral infection with a uropathogenic E. coli strain isolated from a CPPS patient, named CP1 (chronic pain-1), induces chronic inflammation and tactile allodynia in a murine model of the disease. More recently we have also demonstrated that a commensal bacterial strain, named NPI (non-pain inducing) isolated from a healthy control was capable of reducing pain and inflammation in an experimental autoimmune prostatitis murine model. This study focuses on the interplay between these human bacterial strains in the context of pain development and the host immunological response to pathogenic versus commensal colonization. METHODS Male NOD/ShiLtJ mice were inoculated intra-urethrally with either NP1 (S. epidermidis) or CP1 (E. coli), and pelvic tactile allodynia responses measured every seven days by Von Frey Filament behavioral testing. At the experimental endpoint, prostate and bladder tissues were excised and colony formation assays performed. Competitive growth and invasion assays were performed in vitro. Prostate tissue lysates were also used to perform a multiplex luminex bead array to quantify host cytokine and chemokine levels. RESULTS Intra-urethral infection with the pathogenic CP1 induced pelvic tactile allodynia after seven days that became chronic after 14 days. Intra-urethral instillation with the commensal NPI strain seven days prior to infection prevented onset of pelvic tactile allodynia by CP1. Prior instillation with NPI also prevented CP1 colonization of both the prostate and bladder of mice. NPI instillation seven days after infection with CP1 significantly reduced pelvic tactile allodynia. Colony formation assays from prostate and bladder tissues of these mice demonstrated that prior infection CP1 did not significantly reduce the ability of NPI to colonize the prostate. In vitro growth kinetic analysis revealed that CP1 and NPI do not directly inhibit the growth or invasive capacities of each other. Luminex bead array revealed a specific host immune response to single or dual bacterial colonization. CONCLUSIONS Our study demonstrates that commensal bacteria in the human prostate can inhibit the colonisation and pain responses to uropathogenic bacteria, emphasizing the power of the prostatic commensal microflora in controlling health and disease. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e189 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Stephen Murphy More articles by this author Anthony Schaeffer More articles by this author Praveen Thumbikat More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...