MP14-06 RISKS OF CYTOCHROME P450 1B1 POLYMORPHISMS AND LIFESTYLE CHOICES ON PROSTATE CANCER

Abstract

You have accessJournal of UrologyProstate Cancer: Epidemiology & Natural History II1 Apr 2017MP14-06 RISKS OF CYTOCHROME P450 1B1 POLYMORPHISMS AND LIFESTYLE CHOICES ON PROSTATE CANCER Taku Kato, Yutaka Hashimoto, Shigekatsu Maekawa, Marisa Shiina, Mitsuho Imai-Sumida, Pritha Dasgupta, Priyanka Kulkarni, Soichiro Yamamura, Shahana Majid, Sharanjot Saini, Varahram Sharryari, Guoren Deng, Rajvir Dahiya, and Yuichiro Tanaka Taku KatoTaku Kato More articles by this author , Yutaka HashimotoYutaka Hashimoto More articles by this author , Shigekatsu MaekawaShigekatsu Maekawa More articles by this author , Marisa ShiinaMarisa Shiina More articles by this author , Mitsuho Imai-SumidaMitsuho Imai-Sumida More articles by this author , Pritha DasguptaPritha Dasgupta More articles by this author , Priyanka KulkarniPriyanka Kulkarni More articles by this author , Soichiro YamamuraSoichiro Yamamura More articles by this author , Shahana MajidShahana Majid More articles by this author , Sharanjot SainiSharanjot Saini More articles by this author , Varahram SharryariVarahram Sharryari More articles by this author , Guoren DengGuoren Deng More articles by this author , Rajvir DahiyaRajvir Dahiya More articles by this author , and Yuichiro TanakaYuichiro Tanaka More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2017.02.470AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Cytochrome P450 1B1 (CYP1B1) converts xenobiotics to carcinogens and polymorphic variants have been shown to increase activity levels. Lifestyle choices such as tobacco smoking and alcohol consumption are known to enhance the carcinogenesis process and in this study, how these factors may interact with CYP1B1 polymorphisms and affect prostate cancer risk was assessed. METHODS Blood genomic DNA from a Caucasian population consisting of 405 healthy men and 400 prostate cancer patients were obtained. Of these, 507 were current or former smokers and 407 were alcohol drinkers. Eight polymorphic sites of the promoter region of CYP1B1 (rs2551188 G to A, rs2567206 G to A, rs2567207 A to G, rs162556 A to G, rs10175368 C to T, rs163090 T to A, rs162330 T to G, and rs162331 A to G) were analyzed in samples using Taqman genotyping assays and real-time PCR. Lifestyle factors and its influence on CYP1B1 polymorphisms toward cancer risks were also evaluated. RESULTS Overall, both alcohol (P=0.006) and smoker (P=0.069) status were associated with prostate cancer. CYP1B1 variants were also risks for cancer at rs2551188 (P=0.043), rs2567206 (P=0.008), and rs10175368 (P=0.001). Evaluation of linkage disequilibrium show rs2551188, rs2567206, rs2567207, and rs10175368 to be linked and interestingly, the G-G-A-C haplotype (wildtype at respective sites) was significantly reduced in cancer (P=0.0282). When classified by lifestyle factors, no associations for CYP1B1 variants were found for cancer among non-smokers with rs10175368 (P=0.051) being a risk among non-drinkers. On the other hand, variants at both rs2567206 and rs10175368 showed increased cancer risk among smokers (P=0.032 and 0.002, respectively) as well as drinkers (P=0.044 and 0.019, respectively). No genotyping differences were observed when analyzing cancers by pathological grades. CONCLUSIONS These results demonstrate smoker and alcoholic drinker status to modify the risks of CYP1B1 polymorphisms for prostate cancer and this is of importance in understanding their role in the pathogenesis of this disease. © 2017FiguresReferencesRelatedDetails Volume 197Issue 4SApril 2017Page: e164 Advertisement Copyright & Permissions© 2017MetricsAuthor Information Taku Kato More articles by this author Yutaka Hashimoto More articles by this author Shigekatsu Maekawa More articles by this author Marisa Shiina More articles by this author Mitsuho Imai-Sumida More articles by this author Pritha Dasgupta More articles by this author Priyanka Kulkarni More articles by this author Soichiro Yamamura More articles by this author Shahana Majid More articles by this author Sharanjot Saini More articles by this author Varahram Sharryari More articles by this author Guoren Deng More articles by this author Rajvir Dahiya More articles by this author Yuichiro Tanaka More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...