MP12-09 A NOVEL INTRACEREBRAL HEMORRHAGE-INDUCED RAT MODEL OF NEUROGENIC VOIDING DYSFUNCTION: ANALYSIS OF LOWER URINARY TRACT FUNCTION

Abstract

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Basic Research II1 Apr 2015MP12-09 A NOVEL INTRACEREBRAL HEMORRHAGE-INDUCED RAT MODEL OF NEUROGENIC VOIDING DYSFUNCTION: ANALYSIS OF LOWER URINARY TRACT FUNCTION Young Sam Cho, Kwan Joong Joo, Heung Jae Park, and Chil Hun Kwon Young Sam ChoYoung Sam Cho More articles by this author , Kwan Joong JooKwan Joong Joo More articles by this author , Heung Jae ParkHeung Jae Park More articles by this author , and Chil Hun KwonChil Hun Kwon More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.784AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Clinically, stroke is known to be associated with voiding dysfunction. However, lower urinary tract function evaluation in an intracerebral hemorrhage (ICH) model has not been reported. Therefore, we investigated lower urinary tract function in ICH-induced rats and compared the results to those obtained in normal rats. METHODS Adults female Sprague-Dawley rats were divided into two groups: a control group and an ICH-induced group. Induction of ICH in the hippocampal CA1 region was performed using a stereotaxic frame and type IV collagenase. After induction of ICH, we examined the effects of ICH on peripheral bladder function and central micturition centers (medial preoptic area; MPA, ventrolateral gray; PAG, pontaine micturition center; PMC, and spinal cord [L4-L5]). For this study, we simultaneously investigated the effects of ICH on central micturition centers by determining the degree of neuronal activation (c-Fos) and nerve growth factor (NGF) expression and assessing voiding function (urodynamic study using cystometry). RESULTS Induction of ICH significantly enhanced bladder contraction pressure and time, while simultaneously decreasing voiding pressure and time (Fig. 1). Moreover, c-Fos and NGF expression in the neuronal voiding centers was increased by induction of ICH compared to control rats (Fig. 2). CONCLUSIONS We confirmed that ICH clearly induced NLUTD like as other overactive bladder animal models, including a spontaneous hypertensive rat model, a rat model of dopaminergic brain lesions, and a cerebral infarction model. We suggest that our ICH-induced NLUTD rat model is a more appropriate method to study NLUTD in stroke patients than a cerebral infarction model, because the former animal model more accurately reflects the nature of the hemorrhage in both type of stroke. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e131 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Young Sam Cho More articles by this author Kwan Joong Joo More articles by this author Heung Jae Park More articles by this author Chil Hun Kwon More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...