MP11-19 MAGNETIC RESONANCE VISIBILITY OF IRON LABELED MESENCHYMAL STEM CELLS FOR ENDOSCOPIC INJECTION INTO THE PORCINE URETHRAL SPHINCTER

Abstract

You have accessJournal of UrologyImaging/Radiology: Uroradiology II1 Apr 2015MP11-19 MAGNETIC RESONANCE VISIBILITY OF IRON LABELED MESENCHYMAL STEM CELLS FOR ENDOSCOPIC INJECTION INTO THE PORCINE URETHRAL SPHINCTER Susanne Will, Karl-Dietrich Sievert, Martin Vaegler, Petros Martirosian, Frank Eibofner, Fritz Schick, Jörg Schmehl, Gerd Grözinger, Rüdiger Bantleon, Konstantin Nikolaou, and Ulrich Kramer Susanne WillSusanne Will More articles by this author , Karl-Dietrich SievertKarl-Dietrich Sievert More articles by this author , Martin VaeglerMartin Vaegler More articles by this author , Petros MartirosianPetros Martirosian More articles by this author , Frank EibofnerFrank Eibofner More articles by this author , Fritz SchickFritz Schick More articles by this author , Jörg SchmehlJörg Schmehl More articles by this author , Gerd GrözingerGerd Grözinger More articles by this author , Rüdiger BantleonRüdiger Bantleon More articles by this author , Konstantin NikolaouKonstantin Nikolaou More articles by this author , and Ulrich KramerUlrich Kramer More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.400AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES To improve the muscles of the urethral sphincter, different aspects of cell-based treatments should be investigated. Local injection of therapeutic mesenchymal stem cells (MSC) has been proved as a promising approach for tissue regeneration. The aim of this study was to evaluate the effects of superparamagnetic iron oxide (SPIO) cell labeling on the visualization of therapeutic MSC and validate in vitro and in vivo using mini-pig animal models. After injection into the porcine urethral sphincter, the labeled cells were monitored up to six months using MRI. METHODS MSC were labeled with SPIO particles for analysis of migration and viability. Phantom MR measurements were performed in order to evaluate labeling effects. For short and long term studies, iron labeled MSCs were endoscopically injected into the porcine urethral sphincter and monitored on a 3T MRI scanner. The animals were examined three weeks, three and six months after MSC injection. High resolution anatomical images of the urethral sphincter were measured to detect SPIO particles using a fast-spin-echo sequence. A gradient echo sequence with multiple echo times was utilized to visually identify and verify locations of iron particles. The distribution of injected cells was then correlated with histological findings (see Figure). RESULTS Functional analysis of cell parameters after iron labeling showed no effects on proliferation and differentiation of the cells. The adherence increased with a higher iron dose, and the ability to migrate decreased presumably the effect of iron labeling. Measurements of the sphincter muscle in the mini-pig clearly demonstrated visible iron deposits inside the sphincter muscle. Additionally, iron depositions could be verified in all time periods after stem cell injection. The comparison of MRI and tissue sections illustrated preeminent correlation of location and dilatation of iron depots. CONCLUSIONS SPIO-labeled MSC injection into the porcine urethral sphincter and subsequent evaluation with highly resolved MR data acquisition allows sensitive tracking of MSC for several months without any biological effects. Against common knowledge iron deposits within the cells were detectable and correlated histologically even six months after injection. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e126-e127 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Susanne Will More articles by this author Karl-Dietrich Sievert More articles by this author Martin Vaegler More articles by this author Petros Martirosian More articles by this author Frank Eibofner More articles by this author Fritz Schick More articles by this author Jörg Schmehl More articles by this author Gerd Grözinger More articles by this author Rüdiger Bantleon More articles by this author Konstantin Nikolaou More articles by this author Ulrich Kramer More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...