MP10-07 EVALUATION OF BACTERIAL BIOFILMS ON RENAL CALCULI

Abstract

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Kidney & Bladder I1 Apr 2018MP10-07 EVALUATION OF BACTERIAL BIOFILMS ON RENAL CALCULI Negar Motayagheni, Sargurunathan Subashchandrabose, Jorge Gutierrez-Aceves, and Majid Mirzazadeh Negar MotayagheniNegar Motayagheni More articles by this author , Sargurunathan SubashchandraboseSargurunathan Subashchandrabose More articles by this author , Jorge Gutierrez-AcevesJorge Gutierrez-Aceves More articles by this author , and Majid MirzazadehMajid Mirzazadeh More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.353AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Bacterial biofilms are functionally differentiated and organized communities, which have been detected on indwelling devices and in urinary tract infections. The aim of this study is to detect and evaluate bacterial presence and aggregation on uroliths as a potential source of sepsis secondary to stone manipulation. METHODS Urinary stones were collected from patients during PCNL. Samples of uroliths were cultured on LB and MacConkey media under aerobic conditions. Surface bacteria were eliminated with ethanol to delineate the localization of bacteria in renal calculi. Pure cultures of stone-associated bacteria were isolated and species identified with MALDI-TOF mass spectrometry. Stones were dried and coated with gold prior to evaluation by scanning electron microscopy (SEM). Both presence and morphological features of bacterial communities were evaluated. We also reviewed the history of urine cultures and antibiotic therapy within 30 days prior to and during the surgery. RESULTS A total of 23 calculi were obtained from the same number of patients undergoing PCNL. Urine from 8 patients (35%) was culture-positive. Culture of 9 stones (39%) harbored viable Gram-negative bacterial pathogens (Tab 1). Only in one patient (11%), culture of urine and urolith were similar (E. coli). Bacteria were not present in urine of 4 (44%) patients, despite harboring infected calculi. In 7 culture-positive stones (77%), bacterial aggregation was detected by SEM (Fig. 1). The bacterial cells encased in a matrix-like material were suggestive of biofilms. Some stones without biofilm-like structures were colonized by E. coli and P. mirabilis. 4 of the 9 patients (44%) with infected stones were on antibiotics in the month prior to PCNL. Our results do not show correlation between exposure to antibiotics and uroliths infection. CONCLUSIONS Renal calculi colonized with bacteria exhibit morphological features that are reminiscent of bacterial biofilm. Bacteria might be present on the surface or internally within these calculi. There was no correlation between bacterial growth from stone and urine culture. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e118 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Negar Motayagheni More articles by this author Sargurunathan Subashchandrabose More articles by this author Jorge Gutierrez-Aceves More articles by this author Majid Mirzazadeh More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...