MP10-18 FUNCTIONAL ANALYSIS OF THE URINARY TRACT MICROBIOME OF CALCIUM OXALATE STONE FORMERS

Abstract

You have accessJournal of UrologyStone Disease: Basic Research & Pathophysiology II (MP10)1 Apr 2020MP10-18 FUNCTIONAL ANALYSIS OF THE URINARY TRACT MICROBIOME OF CALCIUM OXALATE STONE FORMERS Naveen Kachroo, Caitlin Gold, Manoj Monga, and Aaron Miller* Naveen KachrooNaveen Kachroo More articles by this author , Caitlin GoldCaitlin Gold More articles by this author , Manoj MongaManoj Monga More articles by this author , and Aaron Miller*Aaron Miller* More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000830.018AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Individuals with urinary stone disease (USD) are more likely to take antibiotics in the past and harbor a functionally less diverse microbiome than those with no history of the disease. The urinary tract microbiome of USD patients exhibits greater levels of dysbiosis than the gut microbiome. Therefore, the objective of the current study was to determine what microbial functions of the urinary tract are associated with calcium oxalate (CaOx) formers to understand how the microbiome may contribute to the onset of CaOx stones. METHODS: Two groups of patients, either with no history of USD or an active episode of CaOx stones, were recruited with five patients in each group. The metabolic potential of the urinary tract microbiome was quantified through comparative shotgun metagenomics from clean catch urine samples. Functional differences between groups were assessed through non-parametric t-tests of the normalized abundances of full-length genes, which were annotated through Prodigal and KEGG pathways. Additionally, draft genomes were extracted from the data to aid in future mechanistic studies. RESULTS: Shotgun metagenomics revealed that individuals with CaOx stones had a urinary tract microbiome distinct from those with no history of USD. CaOx stone formers were functionally less diverse than controls, corroborating previous metabolomic studies. Reduced functions of stone formers included oxalate metabolism, previously thought to be relegated to the gut microbiome, as well as transmembrane transport, proteolysis, and oxidation-reduction processes. A total of 17 draft genomes were extracted from the data, primarily from the Lactobacillus genus, which are thought protect against USD and urinary tract infections. CONCLUSIONS: Our prospective clinical study shows that individuals with USD harbor a distinct urinary tract microbiome. Surprisingly, USD patients harbored lower levels of oxalate-degrading genes, which suggests oxalate metabolism may continue in the urinary tract and play a role in inhibiting CaOx stone formation. Additional functions reduced in CaOx stone formers, such as proteolysis and oxidation-reduction processes overlap with known mechanisms of stone formation and thus may also play important roles for the onset of USD. Source of Funding: Lerner Research Institute © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e132-e133 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Naveen Kachroo More articles by this author Caitlin Gold More articles by this author Manoj Monga More articles by this author Aaron Miller* More articles by this author Expand All Advertisement PDF downloadLoading ...