MP1-15 FUNCTIONAL AND MOLECULAR CHARACTERISTIC OF UNDERACTIVE BLADDER INDUCED BY LUMBAR CANAL STENOSIS IN RATS

Abstract

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Basic Research I1 Apr 2014MP1-15 FUNCTIONAL AND MOLECULAR CHARACTERISTIC OF UNDERACTIVE BLADDER INDUCED BY LUMBAR CANAL STENOSIS IN RATS Yao-Chi Chuang, Hung-Jen Wang, Naoki Yoshimura, and Michael Chancellor Yao-Chi ChuangYao-Chi Chuang More articles by this author , Hung-Jen WangHung-Jen Wang More articles by this author , Naoki YoshimuraNaoki Yoshimura More articles by this author , and Michael ChancellorMichael Chancellor More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.113AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES It has been recognized that lumbar canal stenosis (LCS) compromised neurological function is a common problem in elderly patients and may cause underactive bladder (UAB). However, the mechanism and molecular change underlying the UAB is not clear. METHODS One small hole was drilled at the fifth lumbar vertebral arch (sham), and a rectangular piece of silicone rubber was inserted into the L5¡VL6 epidural space (LCS) of female Sprague-Dawley rats. LCS rats underwent bladder expression twice a day. Before and after surgery, a metabolic cage study was performed. Continuous cystometry (CMG) was performed at day 14, or 28 under urethane anesthesia. The bladder was then harvested for histology, expression of M2 and M3 receptors, NGF, and agrin by western blotting. Some animals received intravesical PGE2 instillation. RESULTS LCS induced acute urinary retention and progressive increase of bladder weight. The LCS rats showed a significant decrease in voided volume and a significant increase in postvoid residual urine compared with Sham rats. CMG showed that the LCS bladder was no contraction, high compliance and overflow incontinence. Intravesical PGE2 induced decreased bladder compliance, but no recovery of bladder contraction in the LCS rats. In contrast, there was a significant decrease in intercontraction interval (ICI) and increased in contraction amplitude in response to PGE2 in sham rats. LCS rats showed no obvious difference in detrusor morphology (Fig 1), M2, M3 receptor, NGF, and agrin compared with the sham rats. CONCLUSIONS LCS induced UAB model preserved bladder morphology and functional receptor. It might be a useful model for the mechanism and treatment of neurogenic UAB. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e7 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Yao-Chi Chuang More articles by this author Hung-Jen Wang More articles by this author Naoki Yoshimura More articles by this author Michael Chancellor More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...