MP1-14 POST-STIMULATION INHIBITION OF BLADDER ACTIVITY INDUCED BY TIBIAL NERVE STIMULATION IN RATS

Abstract

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Basic Research I1 Apr 2014MP1-14 POST-STIMULATION INHIBITION OF BLADDER ACTIVITY INDUCED BY TIBIAL NERVE STIMULATION IN RATS Yosuke Matsuta, James Roppolo, William de Groat, Osamu Yokoyama, and Changfeng Tai Yosuke MatsutaYosuke Matsuta More articles by this author , James RoppoloJames Roppolo More articles by this author , William de GroatWilliam de Groat More articles by this author , Osamu YokoyamaOsamu Yokoyama More articles by this author , and Changfeng TaiChangfeng Tai More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.112AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Tibial nerve stimulation (TNS) is useful to treat overactive bladder symptoms. The efficacy of TNS has been reported to be same as antimuscarinics, however, currently the possible mechanisms underlying TNS are not fully understood. The purpose of this study is to develop an animal model for the investigation of the neural mechanisms underlying TNS. METHODS Experiments were conducted in 29 female Sprague-Dawley rats under urethane anesthesia (1.2 g/kg). A catheter was placed into the bladder to perform cystometrogram (CMG). A cuff electrode was placed around right tibial nerve for stimulation. In 24 rats, 3-5 CMGs were performed initially as control CMGs followed by one CMG with simultaneous TNS (5 Hz, 0.2 ms pulse width) at 2 to 4 times the threshold intensity for inducing an observable toe movement. Then, the rats were divided into 2 groups. In the treated group (n=12), TNS was applied for 30 minutes with the bladder empty. In the control group (n=12), the rats were rested for 30 minutes without TNS. Following the 30-minute treatment, 5 CMGs were performed without TNS to evaluate the post-stimulation effect. Bladder capacity (BC), voiding efficacy (VE), maximum voiding pressure (MVP), contraction duration (CD) and bladder compliance were measured. In 5 rats, electromyogram (EMG) was recorded during CMGs to investigate the effect of TNS on external urethral sphincter (EUS) activity. The base-line and the maximal amplitude of EMG activity, bursting duration and frequency during micturition contraction were measured from the EMG recordings. RESULTS TNS applied during CMG had no acute effect on micturition reflex. However, 30-minute-TNS induced significant post-stimulation effects including a 30-40% increase in BC and a 20-30% increase in bladder compliance during the 5 CMGs following the termination of TNS. There was no change in MVP, CD or VE during the following 5 CMGs. TNS did not induce significant changes of EUS EMG activity either during the stimulation or during the post-stimulation period. CONCLUSIONS These results suggest that TNS in rats has no acute effect but has significant post-stimulation inhibitory effect on micturition reflex. TNS has no effect on EUS activity. This animal model will be useful to further investigate the mechanisms underlying the TNS. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e6 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Yosuke Matsuta More articles by this author James Roppolo More articles by this author William de Groat More articles by this author Osamu Yokoyama More articles by this author Changfeng Tai More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...