MP1-07 ENRICHMENT OF CIRCULATING TUMOR CELLS IN PATIENTS WITH LOCALIZED PROSTATE CANCER USING A MICROFLUIDIC DEVICE

Abstract

You have accessJournal of UrologyProstate Cancer: Markers I1 Apr 2015MP1-07 ENRICHMENT OF CIRCULATING TUMOR CELLS IN PATIENTS WITH LOCALIZED PROSTATE CANCER USING A MICROFLUIDIC DEVICE Tilman Todenhöfer, Emily Park, Hamid Abdi, Alex Li, Richard Ross, Xiaoyan Deng, Chao Jin, Simon Duffy, Martin Gleave, Hongshen Ma, and Peter Black Tilman TodenhöferTilman Todenhöfer More articles by this author , Emily ParkEmily Park More articles by this author , Hamid AbdiHamid Abdi More articles by this author , Alex LiAlex Li More articles by this author , Richard RossRichard Ross More articles by this author , Xiaoyan DengXiaoyan Deng More articles by this author , Chao JinChao Jin More articles by this author , Simon DuffySimon Duffy More articles by this author , Martin GleaveMartin Gleave More articles by this author , Hongshen MaHongshen Ma More articles by this author , and Peter BlackPeter Black More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.170AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Circulating tumor cells have proven prognostic value in patients with advanced prostate cancer (PC) but have been identified only infrequently in patients with localized PC using current standard techniques which are dependent on cell surface expression of epithelial cell adhesion molecule (EpCAM). However, evidence suggests that a proportion of CTCs likely do not express EpCAM. We developed a microfluidic system enabling antigen-independent enrichment of circulating tumor cells based on cell size and deformability. The aim of the present study was to evaluate presence of CTCs detected by this platform in patients with clinically localized PC. METHODS We included 58 consecutive patients undergoing radical prostatectomy and pelvic lymphadenectomy for localized PC. As control, we included 7 healthy men. Peripheral blood was collected preoperatively and processed by a microfluidic device for CTC enrichment. This device uses the deformation of single cells through layers of funnel shaped constrictions with openings smaller than the cell diameter in order to selectively transport cells based on size and deformability. Effluent cells were stained for pancytokeratin, CD45 and the androgen receptor (AR), and were identified by spectral analysis on the Zeiss LSM 780 confocal microscope. Cells positive for CK and negative for CD45 were considered to be CTCs. CTC counts were correlated to clinicopathologic parameters. RESULTS One or more CTCs were detected in 0/7 healthy controls and 35/58 (60.3%) patients with PC. The proportions of patients with ≥5 and ≥20 CTCs per ml were 41.4% and 24.1%, and the maximum CTC count was 527 CTCs/ml. We observed no correlation of CTC counts with tumor stage, nodal stage, Gleason score and serum PSA. CTCs were positive for AR-expression. CONCLUSIONS Circulating tumor cells can be detected in a considerable proportion of patients with localized prostate cancer using an EpCAM-independent system. CTC counts in our study were clearly higher than in other cohorts using EpCAM-dependent detection. The expression of AR in cytokeratin positive CTCs confirms their prostatic origin. The prognostic value of CTCs in peripheral blood before and after prostatectomy remains to be defined. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e3 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Tilman Todenhöfer More articles by this author Emily Park More articles by this author Hamid Abdi More articles by this author Alex Li More articles by this author Richard Ross More articles by this author Xiaoyan Deng More articles by this author Chao Jin More articles by this author Simon Duffy More articles by this author Martin Gleave More articles by this author Hongshen Ma More articles by this author Peter Black More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...