MP09-09 BRAIN NITRIC OXIDE CAN INDUCE FREQUENT URINATION IN RATS

Abstract

You have accessJournal of UrologyBladder & Urethra: Anatomy, Physiology & Pharmacology I1 Apr 2018MP09-09 BRAIN NITRIC OXIDE CAN INDUCE FREQUENT URINATION IN RATS Takahiro Shimizu, Hideaki Ono, Shogo Shimizu, Youichirou Higashi, Suo Zou, Masaki Yamamoto, Takaaki Aratake, Tomoya Hamada, Yoshiki Nagao, Yusuke Ueba, Masashi Honda, and Motoaki Saito Takahiro ShimizuTakahiro Shimizu More articles by this author , Hideaki OnoHideaki Ono More articles by this author , Shogo ShimizuShogo Shimizu More articles by this author , Youichirou HigashiYouichirou Higashi More articles by this author , Suo ZouSuo Zou More articles by this author , Masaki YamamotoMasaki Yamamoto More articles by this author , Takaaki AratakeTakaaki Aratake More articles by this author , Tomoya HamadaTomoya Hamada More articles by this author , Yoshiki NagaoYoshiki Nagao More articles by this author , Yusuke UebaYusuke Ueba More articles by this author , Masashi HondaMasashi Honda More articles by this author , and Motoaki SaitoMotoaki Saito More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.335AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Nitric oxide (NO) has a local inhibitory effect on urination. While in the central nervous system, NO seems to have both inhibitory and facilitatory effects on micturition. We previously reported that an NO donor SIN-1 centrally activated the sympatho-adrenomedullary (SA) system in rats. In this study, we investigated effects of centrally administered SIN-1 on micturition and their dependence on the SA system in rats. METHODS Urethane anesthetized (0.8-1.0 g/kg, ip) male Wistar rats (300-400 g) were used. (1) Catheters were inserted into the bladder and the femoral artery in order to perform continuous cystometrograms (CMG, 12 ml/h saline infusion) and to collect blood samples, respectively. Three hours after the surgery, SIN-1 (100 or 250 μg/rat) or vehicle was icv administered. Saline infusion into the bladder was started 1 hour before the icv administration. Plasma noradrenaline (NA) and adrenaline (Ad) levels were measured at 5 min after the icv administration. In some experiments, carboxy-PTIO (PTIO, an NO scavenger, 750 μg/rat) was icv pretreated 30 min before icv SIN-1 administration. (2) Three hours after the surgery of a bladder catheter insertion, single CMG (12 ml/h saline infusion) was performed. After 4-5 times of single CMG, SIN-1 (250 μg/rat) was icv administered, then single CMG was continued for 1 hour. RESULTS (1) SIN-1 dose-dependently reduced intercontraction intervals (ICI) and elevated plasma Ad without altering maximal voiding pressure or plasma NA (Tables 1-2). An insufficient dose of SIN-1 (100 μg/rat) for elevating plasma AD could induce ICI reduction (Tables 1-2). These SIN-1-induced responses were inhibited by PTIO (Table 3). (2) SIN-1 reduced single-voided volume (Vv) and bladder capacity (BC) without altering post-voiding residual urine volume or voiding efficiency (Table 4). CONCLUSIONS NO centrally induces frequent urination shown by reduced ICI, Vv and BC, independent of the central SA outflow. Thus, the brain nitrergic pathway that is directly involved in the control of micturition could be a new target for the treatment of bladder dysfunction. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e109 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Takahiro Shimizu More articles by this author Hideaki Ono More articles by this author Shogo Shimizu More articles by this author Youichirou Higashi More articles by this author Suo Zou More articles by this author Masaki Yamamoto More articles by this author Takaaki Aratake More articles by this author Tomoya Hamada More articles by this author Yoshiki Nagao More articles by this author Yusuke Ueba More articles by this author Masashi Honda More articles by this author Motoaki Saito More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...