MP08-08 NERVE TRANSFER FOR RESTORATION OF LOWER MOTOR NEURON-LESIONED BLADDER FUNCTION. PART 2: ATTENUATION OF PURINERGIC BLADDER SMOOTH MUSCLE CONTRACTIONS

Abstract

You have accessJournal of UrologyBladder & Urethra: Anatomy, Physiology & Pharmacology (MP08)1 Sep 2021MP08-08 NERVE TRANSFER FOR RESTORATION OF LOWER MOTOR NEURON-LESIONED BLADDER FUNCTION. PART 2: ATTENUATION OF PURINERGIC BLADDER SMOOTH MUSCLE CONTRACTIONS Nagat Frara, Dania Giaddui, Alan S. Braverman, Danielle S. Porecca, Justin M. Brown, Michael Mazzei, Ida J. Wagner, Michel A. Pontari, Ekta Tiwari, Courtney L. Testa, Daohai Yu, Locas J. Hobson, Mary F. Barbe, and Michael R. Ruggieri Nagat FraraNagat Frara More articles by this author , Dania GiadduiDania Giaddui More articles by this author , Alan S. BravermanAlan S. Braverman More articles by this author , Danielle S. PoreccaDanielle S. Porecca More articles by this author , Justin M. BrownJustin M. Brown More articles by this author , Michael MazzeiMichael Mazzei More articles by this author , Ida J. WagnerIda J. Wagner More articles by this author , Michel A. PontariMichel A. Pontari More articles by this author , Ekta TiwariEkta Tiwari More articles by this author , Courtney L. TestaCourtney L. Testa More articles by this author , Daohai YuDaohai Yu More articles by this author , Locas J. HobsonLocas J. Hobson More articles by this author , Mary F. BarbeMary F. Barbe More articles by this author , and Michael R. RuggieriMichael R. Ruggieri More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000001981.08AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: This study determined the effect of pelvic organ long-term decentralization and reinnervation one year later on the contribution of muscarinic and purinergic receptors to ex-vivo, nerve-evoked, bladder smooth muscle contractions. METHODS: Nineteen canines underwent decentralization by bilateral transection of all coccygeal and sacral (S) spinal roots, dorsal roots of lumbar (L)7 and hypogastric nerves. After exclusions, 8 were reinnervated 12 months post-decentralization with obturator-to-pelvic and sciatic-to-pudendal nerve transfers then euthanized 8-12 months later; four served as long-term decentralized only animals. Controls included six sham-operated and three unoperated animals. Bladder tissues were assessed for contractile responses to potassium chloride (KCl) and electric field stimulation (EFS) before and after purinergic receptor desensitization with alpha, beta-methylene adenosine triphosphate (α,β-mATP), muscarinic receptor antagonism with atropine, or sodium channel blockade with tetrodotoxin. RESULTS: Atropine inhibition of EFS-induced contractions increased by 26%in decentralized and 34%in the reinnervated animals compared to controls. Maximal contractile responses to α,β-mATP did not differ between groups. In strips from decentralized and reinnervated animals, the contractile response to EFS was enhanced by 52% at lower frequencies and the response to KCl was increased compared to normal controls. CONCLUSIONS: The observation of increased blockade of nerve-evoked contractions by muscarinic antagonist with no change in responsiveness to purinergic agonist suggests either decreased ATP release or increased ectoATPase activity in detrusor muscle as a consequence of the long-term decentralization. The reduction in the frequency required to produce maximum contraction following decentralization may be due to enhanced nerve sensitivity to EFS or a change in the effectiveness of the neurotransmission. Source of Funding: NINDS R01NS070267 © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e153-e153 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information Nagat Frara More articles by this author Dania Giaddui More articles by this author Alan S. Braverman More articles by this author Danielle S. Porecca More articles by this author Justin M. Brown More articles by this author Michael Mazzei More articles by this author Ida J. Wagner More articles by this author Michel A. Pontari More articles by this author Ekta Tiwari More articles by this author Courtney L. Testa More articles by this author Daohai Yu More articles by this author Locas J. Hobson More articles by this author Mary F. Barbe More articles by this author Michael R. Ruggieri More articles by this author Expand All Advertisement Loading ...