MP07-05 THE MECHANISM OF KIDNEY STONE SUPPRESSION BY PROLINE HYDROXYLASE INHIBITOR ADMINISTRATION

Abstract

You have accessJournal of UrologyStone Disease: Basic Research & Pathophysiology (MP07)1 Sep 2021MP07-05 THE MECHANISM OF KIDNEY STONE SUPPRESSION BY PROLINE HYDROXYLASE INHIBITOR ADMINISTRATION Kengo Kawase, Shuzo Hamamoto, Tatsuya Hattori, Tomoki Okada, Yutaro Tanaka, Takeru Sugino, Rei Unno, Kazumi Taguchi, Ryosuke Ando, Atsushi Okada, and Takahiro Yasui Kengo KawaseKengo Kawase More articles by this author , Shuzo HamamotoShuzo Hamamoto More articles by this author , Tatsuya HattoriTatsuya Hattori More articles by this author , Tomoki OkadaTomoki Okada More articles by this author , Yutaro TanakaYutaro Tanaka More articles by this author , Takeru SuginoTakeru Sugino More articles by this author , Rei UnnoRei Unno More articles by this author , Kazumi TaguchiKazumi Taguchi More articles by this author , Ryosuke AndoRyosuke Ando More articles by this author , Atsushi OkadaAtsushi Okada More articles by this author , and Takahiro YasuiTakahiro Yasui More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000001980.05AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Kidney stones are associated with metabolic syndrome (MetS). MetS has been shown to create a hypoxic environment in adipose tissue, resulting in inflammation. It has been reported that the prolyl hydroxylases (PHD)-hypoxia-inducible factor (HIF) pathway is mainly involved in the hypoxic response and that PHD inhibitors suppress inflammation in adipose tissue. In this study, we investigated the inhibitory effect of a PHD inhibitor (FG) on kidney stone formation. METHODS: A group of cultured mouse renal tubular cells (M1) and a group of co-cultured M1 and 3T3L1 cells differentiated into white adipocytes were prepared. FG (0 μmol/L, 50 μmol/L) was added to all for 6 hours, and calcium oxalate crystals (20 μg/cm2) were added to M1 for a total of four groups. The extent of crystal adhesion was examined. In addition, inflammation and stress were determined by quantitative PCR using Ccl2 and Spp1, and the expression of Adiponectin in the culture medium were evaluated. RESULTS: FG treatment significantly decreased the amount of crystal adherence in both monoculture and co-culture groups (p <0.05), and FG treatment significantly decreased the expression of Ccl2 and Spp1 in M1 cells in both monoculture and co-culture groups (p <0.05). Adiponectin in culture was increased in the co-culture group by FG treatment (p <0.05). CONCLUSIONS: The activation of HIF in adipocytes by FG administration was suggested to inhibit renal stone formation by showing adiponectin-mediated anti-inflammatory effects. Source of Funding: The authors declare no conflicts of interest associated with this manuscript © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e140-e141 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information Kengo Kawase More articles by this author Shuzo Hamamoto More articles by this author Tatsuya Hattori More articles by this author Tomoki Okada More articles by this author Yutaro Tanaka More articles by this author Takeru Sugino More articles by this author Rei Unno More articles by this author Kazumi Taguchi More articles by this author Ryosuke Ando More articles by this author Atsushi Okada More articles by this author Takahiro Yasui More articles by this author Expand All Advertisement Loading ...