MP01-19 STRUCTURE ESTABLISHMENT OF THREE-DIMENSIONAL (3D) CELL CULTURE PRINTING MODEL FOR BLADDER CANCER

Abstract

You have accessJournal of UrologyBladder Cancer: Basic Research & Pathophysiology I (MP01)1 Apr 2020MP01-19 STRUCTURE ESTABLISHMENT OF THREE-DIMENSIONAL (3D) CELL CULTURE PRINTING MODEL FOR BLADDER CANCER Se Young Choi, Jae Hun Shim, Byung Hoon Chi, Jin Wook Kim, Seung Hyun Ahn, Tae-Hyoung Kim*, Soon Chul Myung, Kyung Do Kim, and In Ho Chang Se Young ChoiSe Young Choi More articles by this author , Jae Hun ShimJae Hun Shim More articles by this author , Byung Hoon ChiByung Hoon Chi More articles by this author , Jin Wook KimJin Wook Kim More articles by this author , Seung Hyun AhnSeung Hyun Ahn More articles by this author , Tae-Hyoung Kim*Tae-Hyoung Kim* More articles by this author , Soon Chul MyungSoon Chul Myung More articles by this author , Kyung Do KimKyung Do Kim More articles by this author , and In Ho ChangIn Ho Chang More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000815.019AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Two-dimensional (2D) cell culture is a valuable method for cell-based research but can provide unpredictable, misleading data about in vivo responses. In this study, we created a three-dimensional (3D) cell culture environment to mimic tumor characteristics and cell-cell interactions to better characterize the tumor formation response to chemotherapy. METHODS: We fabricated the 3D cell culture samples using a 3D cell bio printer and the bladder cancer cell line 5637. T24 cells were used for 2D cell culture. Then, rapamycin and Bacillus Calmette-Guérin (BCG) were used to examine their cancer inhibition effects using the two bladder cancer cell lines. Cell-cell interaction was measured by measuring e-cadherin and n-cadherin secreted via the epithelial-mesenchymal transition (EMT). RESULTS: We constructed a 3D cell scaffold using gelatin methacryloyl (GelMA) and compared cell survival in 3D and 2D cell cultures. 3D cell cultures showed higher cancer cell proliferation rates than 2D cell cultures, and the 3D cell culture environment showed higher cell-to-cell interactions through the secretion of E-cadherin and N-cadherin. Assessment of the effects of drugs for bladder cancer such as rapamycin and BCG showed that the effect in the 2D cell culture environment was more exaggerated than that in the 3D cell culture environment. CONCLUSIONS: We fabricated 3D scaffolds with bladder cancer cells using a 3D bio printer, and the 3D scaffolds were similar to bladder cancer tissue. This technique can be used to create a cancer cell-like environment for a drug screening platform. Source of Funding: None © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e9-e10 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Se Young Choi More articles by this author Jae Hun Shim More articles by this author Byung Hoon Chi More articles by this author Jin Wook Kim More articles by this author Seung Hyun Ahn More articles by this author Tae-Hyoung Kim* More articles by this author Soon Chul Myung More articles by this author Kyung Do Kim More articles by this author In Ho Chang More articles by this author Expand All Advertisement PDF downloadLoading ...