MP01-04 A NEW SEMEN CONTAINER DESIGNED TO MINIMIZE DAMAGE TO SPERM DURING TRANSPORTATION AT LOW TEMPERATURE CONDITIONS

Abstract

You have accessJournal of UrologyCME1 Apr 2023MP01-04 A NEW SEMEN CONTAINER DESIGNED TO MINIMIZE DAMAGE TO SPERM DURING TRANSPORTATION AT LOW TEMPERATURE CONDITIONS Hiroshi Okada, Toshiyuki Iwahata, Hiroshi Okada, Hiroshi Okada, Kazutaka Saito, and Kouhei Sugimoto Hiroshi OkadaHiroshi Okada More articles by this author , Toshiyuki IwahataToshiyuki Iwahata More articles by this author , Hiroshi OkadaHiroshi Okada More articles by this author , Hiroshi OkadaHiroshi Okada More articles by this author , Kazutaka SaitoKazutaka Saito More articles by this author , and Kouhei SugimotoKouhei Sugimoto More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003212.04AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Rapid cooling is believed to be harmful to mammalian spermatozoa. Thus, special care should be taken when transporting semen in low temperature conditions. A new semen container, TPS-2 was designed to protect sperm against damage caused by cold shock. We investigated the ability of TPS-2 to protect sperm from low temperature conditions, using sperm from both fertile and infertile men. METHODS: We measured the change in temperature of semen stored in TPS-2 at 10°C compared with Sperm Cup, a conventional semen collecting cup. We also investigated change in total sperm motility, sperm DNA fragmentation index, and seminal oxidation-reduction potential when stored in TPS-2 or Sperm Cup at 10°C. We used Sperm Cup and TPS-2 to transport 25 semen samples of infertile patients from home to the clinic below 10°C, and compared the difference of sperm motion parameters. RESULTS: After storage for 3 h, the temperature in TPS-2 and Sperm Cup was 20.1°C and 12.1°C, respectively (Figure 1). The temperature dropped more gradually in TPS-2 than in Sperm Cup. Semen from fertile volunteers stored in Sperm Cup showed significantly lower total sperm motility, whereas in TPS-2, total sperm motility was the same as at 25°C (Figure 2). Semen from infertile patients stored at 10°C showed significantly lower total sperm motility in Sperm Cup. In TPS-2, total sperm motility did not change after storage at 10°C for 3 h. TPS-2 could maintain sperm motility (p=0.000), percentage of rapid moving sperm (p=0.002) and total motile sperm count (p=0.014) during transportation in low temperature conditions (Figure 3). CONCLUSIONS: TPS-2 maintains the temperature of semen stored in it during exposure to low temperatures conditions, preventing sperm damage. TPS-2 is recommended for transportation of semen in cold conditions. Source of Funding: None © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e2 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Hiroshi Okada More articles by this author Toshiyuki Iwahata More articles by this author Hiroshi Okada More articles by this author Hiroshi Okada More articles by this author Kazutaka Saito More articles by this author Kouhei Sugimoto More articles by this author Expand All Advertisement PDF downloadLoading ...