MoYcp4 is required for growth, conidiogenesis and pathogenicity in Magnaporthe oryzae.

Abstract

The transcription factor MoAP1 has been shown previously to be required for pathogenicity in Magnaporthe oryzae via mediation of the oxidative stress response. In the serial analysis gene expression database, it was found that expression of MoYcp4, a homologue of the Saccharomyces cerevisiae flavodoxin-like protein ScYcp4, was affected by MoAP1. Transcriptional analysis demonstrated that MoYCP4 was significantly up-regulated during conidiation, appressorium formation and infection. The growth rate of a ΔMoycp4 mutant was reduced slightly, but conidial production was increased significantly (more than 10-fold), compared with the wild-type strain. Although the rate of appressorium formation was unaffected, the appressorial turgor was abnormal and the ability to infect rice and barley was reduced, resulting in decreased pathogenicity. In summary, MoYcp4, a target of MoAP1, is involved in the growth, conidiogenesis and pathogenicity of M. oryzae. Our studies provide a comprehensive analysis of flavodoxin-like proteins and will aid in the study of pathogen-related molecular mechanisms.