Mouse resident lung eosinophils are dependent on IL-5.

Abstract

Eosinophils were traditionally considered terminally differentiated cells with little to no heterogeneity.1 Recent studies demonstrated that they can acquire unique phenotypes in response to diverse stimuli,1-3 and two subsets of eosinophils [i.e. resident (rEos) and inflammatory (iEos)] were described in the lungs of allergen-challenged mice.4 rEos and iEos can be differentiated by the expression of Siglec-F, CD101 and CD62L, their functional activities and their dependency on IL-5.4 In particular, survival of lung rEos, which were associated with immunoregulatory activities was described as IL-5-independent.4 This distinction may have major therapeutic implications since neutralization of IL-5 via anti-IL-5 therapy may deplete iEos and keep rEos intact.5 Conversely, depletion of eosinophils using antibody-dependent cell cytotoxicity via targeting anti-IL-5Rα (e.g. benralizumab) may deplete both populations. Yet, experience in humans has not revealed adverse consequences nor distinct residual eosinophil populations following anti-IL-5 therapeutics.1 Herein, we demonstrate that rEos require IL-5 for their survival and that the expression of Siglec-F, is regulated by IL-5 drawing caution to previous conclusions.4 Wild type (WT) mice were challenged with house dust mite, and the presence of lung eosinophils was determined (see Methods S1). At baseline, one population of lung rEos (Siglec-Fint/CD101−) were identified (Figure 1A-B). We could not identify the expression of CD62L, a suggested marker of resident lung eosinophils4 in any eosinophil population (not shown). On day 10, two eosinophil populations, which appeared to correspond with the previously identified rEos and iEos (e.g. Siglec-Fint and Siglec-Fhi) were observed (Figure 1A-B). However, by day 21 most lung eosinophils consisted only of Siglec-Fhi cells and expressed CD101 (Figure 1A-D). This suggested that an environmental factor, such as IL-5 regulates Siglec-F expression. Certainly, IL-5 increased the expression of Siglec-F in a concentration-dependent fashion on the surface of peritoneal eosinophils obtained from WT mice (Figure 1E). Similarly, eosinophils from the bone marrow, blood, spleen and lungs of Il5Tg mice displayed elevated levels of Siglec-F expression (Figure 1F-I). Administration of anti-IL-5 neutralizing antibodies to naïve and day 10 allergen-challenged mice, which have Siglec-Fint and Siglec-Fhi eosinophil populations (Figure S1) decreased the expression of Siglec-F the peripheral blood and lung eosinophils from naïve mice (Figure 1J-M) as well as in blood, lungs and bronchoalveolar lavage fluid eosinophils from allergen-challenged mice (Figure 1N-S). Conversely, administration of IL-5 to the peritoneal cavity of WT mice, increased the expression of Siglec-F in peritoneal eosinophils (Figure 1T-U). These data suggest that Siglec-Fhi eosinophils in the lungs of allergen-challenged mice (Figure 1A) may comprise a mixture of rEos and iEos. IL-5 neutralization in naïve mice markedly decreased the percentage and total numbers of resident lung and peripheral blood eosinophils (Figure 2A-B). Furthermore, IL-5 neutralization decreased the levels of blood, lung and BALF eosinophils in allergen-challenged mice (Figure 2C-D). Transcriptional profiling of lung rEos, allergen-challenged Siglec-Fint/CD101− and Siglec-Fhi/CD101+ eosinophils revealed that lung rEos clustered with allergen-challenged Siglec-Fint/CD101− cells whereas allergen-challenged Siglec-Fhi/CD101+ cells were distinct. Horizontal clustering demonstrated three main clusters that were unique for each population (Figure 2E, Tables S1). rEos were enriched with pathways that were associated with cell defense and innate immunity whereas allergen-challenged Siglec-Fint/CD101− eosinophils were enriched with metabolic pathways suggesting an active cellular state6 (Figure 2F-H). Venn-plot analysis revealed that allergen-challenged SiglecFhi/CD101+ eosinophils uniquely upregulate 234 and 565 transcripts in comparison with rEos and allergen-challenged SiglecFint cells, respectively (Figure 2I, Tables S2-S3). Allergen-challenged SiglecFint/CD101− eosinophils uniquely upregulated 77 transcripts in comparison with resident eosinophils (Figure 2I, Table S4). The study bears the limitation that it was conducted in mice, which display additional IL-5R+ cells (e.g. neutrophils) and mouse eosinophils differ from human eosinophils. Nonetheless, these data show that anti-IL-5 treatment downregulates Siglec-F and depletes all lung populations of eosinophils. This is inconsistent with rEos being independent of IL-5, and further suggests that distinct eosinophil populations in the asthmatic lung represent a continuum of activation states rather than different cellular subsets. AD and AM involved in conception and/or design of the work. AD, SGT, SA, IH, YG and MI assisted with data collection. AD, SGT and AM performed data analysis and interpretation. AD and AM involved in drafting the article. SGT, SA, IH, YG and MI involved in critical revision of the article. AM involved in final approval of the version to be published. The authors wish to thank the members of the Munitz laboratory for their fruitful discussions. A.M received grants from AstraZeneca and GlaxoSmithKline, and personal fees from AstraZeneca, Sanofi and GlaxoSmithKline. The other authors declare no relevant conflicts of interest. 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