Mouse Renal Artery Catheterization for Local Delivery of Drugs in Capsulated or Free Forms.

Abstract

Arterial delivery to the kidney offers significant potential for targeted accumulation and retention of cells, genetic material, and drugs, both in free and encapsulated forms, because the entire dose passes through the vessels feeding this organ during the first circulation of blood. At the same time, a detailed study on the safety and effectiveness of developed therapies in a large number of experimental animals is required. Small laboratory animals, especially mice, are the most sought-after in experimental and preclinical testing due to their cost-effectiveness. Most of the described manipulations in mice involve puncturing the walls of the abdominal aorta or renal artery for direct administration of solutions and suspensions. Such manipulations are temporary and, in some cases, result in long-term occlusion of the aorta. Ultimately, this can lead to disruption of blood flow as well as functional and morphological changes to the kidneys. In addition, few of these protocols describe targeted delivery to the kidney. The presented protocol involves the injection of test substances or suspensions through the renal artery into one of the kidneys. The catheter is implanted into the femoral artery and then advanced into the abdominal aorta and renal artery within the vessels. In this case, the integrity violation of the renal artery or abdominal aorta is absent. Occlusion of the renal artery is necessary only immediately at the time of injection to minimize the entry of the injected substance into the aorta. This protocol is similar to the clinical procedure for delivering a catheter into the renal artery and is designed for real-world operating conditions. Key features • The protocol involves implantation of a catheter into the vascular system through a puncture of the femoral artery, similar to the clinical procedure. • The catheter is moved inside the vessels without puncture or ligation to the aorta or renal artery. • The protocol involves only a short-term block of the blood supply to the target kidney (the time required for direct administration of the drug). • Suitable for chronic experiments on mice, since the catheter is removed from the vascular system immediately after drug administration.