Abstract
Our previous study showed that water immersion restraint stress (WRS) induced osteopenia. Since relationship between bone mineral density (BMD) and muscle amount was reported, histochemical analysis of soleus was performed after WRS.WRS was performed on 8 week old male C57/6J mice 6h/day, 5 times/week for 4 weeks. After sacrifice, soleus was isolated and histochemical analysis was performed.Although no growth retardation was observed, both muscle amount and grip strength were decreased in WRS group. In various types of muscle fibers, histochemical analysis of soleus showed that areas of type 2b muscle fiber was significantly decreased in WRS group. Serum corticosterone level was significantly higher in WRS group, indicating that WRS was stressful to mice. mRNA expressions of atrogin‐1 and MuRF‐1 were significantly higher in WRS group, although myostatin and myogenin mRNA expressions were not changed. These results indicated that WRS might induce ubiquitin‐proteasome dependent protein degradation, and resulting muscle atrophy.
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