Abstract
Atopic dermatitis is a skin disease characterized by allergic skin inflammation, redness and itching. The animal model is necessary to find out new drugs. The DNCB-induced animal model of atopic dermatitis includes the following steps: 1) Selection of animals; 2) Shaving of dorsal skin; 3) Applying DNCB once in 24 hours for three days; 4) Monitoring the development of atopy on day 4 post DNCB application. Further, the efficacy of reference drug can be determined by applying on the atopy skin, depends on the nature and aim of the work.Video Clip of Methodology: 8 min Full Screen Alternate
Highlights
Atopic dermatitis is a chronic allergic disease of the skin characterized by inflammation and rashes
DNCB (1% and 2%): Dissolve 100 mg DNCB powder in 20 mL of acetone/olive oil (4:1; v/v) solution (2%)
After two weeks of adaptation period, mice were subjected to induction of DNCB-induced atopic dermatitis
Summary
Atopic dermatitis is a chronic allergic disease of the skin characterized by inflammation and rashes. In >80% of the cases in children, the serum IgE level is increased which is due to the impaired balance of the CD4-positive T-helper cell populations Th1 and Th2 with a predominance of IL-4 and IL-13 producing Th2 cells This disease does not have any permanent or long-term treatment. A large number of new drugs are synthesized but fail to establish in the clinical trials Most of these failures are due to a variety of reasons, including inadequate efficacy, safety and tolerability. You must attribute the work in the manner specified by the author or licensor
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