Abstract
Binding strength to protein A discriminates three definitive types of mouse monoclonal IgG 1. Each of these types of IgG 1 elutes from protein A-Sepharose as a coherent peak representing the great majority of IgG 1 protein. One type of IgG 1 is not present to any significant extent in polyclonal mouse IgG preparations, and to our knowledge has not been reported previously. This type of IgG 1 does not bind firmly to protein A at pH 8.0, but is retarded sufficiently that it can be purified by protein A-Sepharose chromatography in high yields.
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