Abstract
The present study is a report on the use of individual micro fans attached to the lids of mice cages, leading to cage pressurization and internal ventilation. The effect of this ventilation system was analyzed in relation to reproductive performance and lung histopathology of BALB/c, SPF mice, and was based on the results of previous studies, which demonstrated the mechanical efficiency of the system. No statistical differences were observed in relation to interbirth interval, number of offspring born, and number and weight of offspring weaned. Lung histopathology, however, showed that the group of animals kept under the microenvironmental ventilation system for 229 days presented no pulmonary lesions (score 0), whereas animals in the control group, kept in a conventional micro isolator, without ventilation, presented score 1 (discrete inflammatory reaction and hemorrhage, 5 animals); score 2 (hemorrhage, 1 animal) and score 3 (interstitial pneumonia and hemorrhage or peribronchitis and hemorrhage, 2 animals). Data demonstrates that the presence and functioning of the micro fans on the lids did not interfere with the reproductive performance of the animals and improve the quality in laboratory animals production, providing them without any kind of pulmonary lesions.
Highlights
Several studies [1,2,3,4,5,6] have demonstrated the efficiency of microenvironmental ventilation systems (MV) [7] for laboratory animals, these systems are more efficient than
Based on results and improvements obtained using PIV, the objective of the present trial was to assess the reproductive performance and to quantify pulmonary lesions observed in mice kept under a microenvironmental ventilation system using micro fans
No statistical differences were observed in interbirth interval (Table 1)
Summary
Several studies [1,2,3,4,5,6] have demonstrated the efficiency of microenvironmental ventilation systems (MV) [7] for laboratory animals, these systems are more efficient thanGeneral Diluting Ventilation (GDV) ones, which were previously recommended for this purpose [8,9,10,11,12]. The use of individual micro fans attached to the lid of the cages (pressurized intraventilated—PIV), has recently been introduced showing a low cost solution and smaller ammonia concentrations in cages microenvironment [23,24] This alternative is based on the use of direct-current micro fans for ventilating and pressurizing these compartments. Previous experiments demonstrated that micro fans similar to those employed as conventional desktop computers cooling fans are suitably adaptable to voltage fluctuations, allowing for precise airflow control In addition to their low implementation costs, micro fans are available on the market, require little or almost no maintenance, and have an expected mean lifespan of 20,000 hours under constant operation. Based on results and improvements obtained using PIV, the objective of the present trial was to assess the reproductive performance and to quantify pulmonary lesions observed in mice kept under a microenvironmental ventilation system using micro fans
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