Abstract
Abstract A valuable model for studying virus-host interactions is mouse cytomegalovirus (MCMV), which encodes the m145 family of glycoproteins with predicted MHC-I structure. m153, expressed independently of beta-2 microglobulin and peptide, and is found on the surface of infected cells by flow cytometry. We constructed a reporter cell to identify tissues expressing the ligand of m153 by preparing the DNA encoding the extracellular domains of m153 linked to human zeta chain, and introducing this construct into 43.1 cells that fluoresce green on activation. We identified CD11c+ splenic dendritic cells (DC) and bone marrow DC as potent stimulators. Further characterization of these cells via multi-color staining with an m153 tetramer, and DC markers and antibody blocking experiments suggest a possible candidate for the m153 ligand. This may contribute to our understanding of the steps in virus lifecycle, and shed light on the evolution of viral immunoevasins.
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