Abstract
PurposeTo develop a tissue fixation method that preserves in vivo manganese enhancement for ex vivo magnetic resonance imaging (MRI). The needs are clear, as conventional in vivo manganese‐enhanced MRI (MEMRI) applied to live animals is time‐limited, hence limited in spatial resolution and signal‐to‐noise ratio (SNR). Ex vivo applications can achieve superior spatial resolution and SNR through increased signal averaging and optimized radiofrequency coil designs. A tissue fixation method that preserves in vivo Mn2+ enhancement postmortem is necessary for ex vivo MEMRI.Materials and MethodsT1 measurements and T1‐weighted MRI were performed on MnCl2‐administered mice. The mice were then euthanized and the brains were fixed using one of two brain tissue fixation methods: aldehyde solution or focused beam microwave irradiation (FBMI). MRI was then performed on the fixed brains.ResultsT1 values and T1‐weighted signal contrasts were comparable between in vivo and ex vivo scans on aldehyde‐fixed brains. FBMI resulted in the loss of Mn2+ enhancement.ConclusionAldehyde fixation, not FBMI, maintained in vivo manganese enhancement for ex vivo MEMRI. J. Magn. Reson. Imaging 2013;38:482–487. © 2013 Wiley Periodicals, Inc.
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